Three Luminous Detection Methods of Luminol

 

Luminol is a common chemiluminescence reagent, which is mainly used in the immunochemiluminescence system and criminal detection field. It also plays an important role in the detection of sexual hormones, drug abuse, and cardiovascular and reproductive system diseases. Luminol has three main luminescence detection methods in practical applications.

 

1. Adding catalyst to accelerate luminescence:

Adding catalyst is a common method at present. Common catalysts in luminol luminescence system include metal complexes (such as hemoglobin, hydrogen peroxide, etc.), transition metal ions (such as Fe3+, Fe2+, Mn2+, Cr2+, etc.), and peroxides (horseradish peroxidase, etc.). The properties and weight of these catalysts will not change after the completion of the luminescence reaction. Even if the concentration of the catalyst is too high, it will be detected, so it will not affect the detection results.

 

2. Indirect determination by adding inhibitor:

In addition to catalysts that can promote luminol's luminescence, relevant researchers have found that some substances can inhibit luminol's luminescence intensity. For example, reducing compounds containing phenolic hydroxyl groups, also known as inhibitors, can react with oxidants, reduce the concentration of oxidants, reduce the intensity of chemiluminescence, and indirectly determine such organic substances.

 

3. Over-coupling indirect determination method:

The third is coupling reaction for indirect determination. Coupling reaction refers to the combination of one reaction that can produce or consume chemiluminescence reactants with another chemiluminescence reaction, so as to realize the indirect chemiluminescence determination of some substances. For example, some substrates produce hydrogen peroxide under the action of some enzymes and then produce chemiluminescence with luminol. By measuring chemiluminescence, we can indirectly know the purity of the measured substrate.

 

Hubei Xindesheng Material Technology Co., Ltd. has specialized in the research and development of chemiluminescent reagents for 18 years, and has cooperated with more than 400 enterprises at home and abroad. The spirit of continuous pursuit of excellent quality is widely recognized by users at home and abroad. In addition to Luminol, acridine ester series products are available in stock. If you also have relevant purchase requirements, please contact me or click the official website for inquiry!