Comparison of TRIS and HEPES in biochemical application and skin care

 

TRIS and HEPES are two widely used chemical reagents. In addition to being used as biological buffers, they are also commonly used as mild and safe cosmetics additives. Understanding the differences between them can make them more accurate in use and avoid unnecessary mistakes.

 

1. Comparison of biochemical application

TRIS (trimethylolaminomethane) is weakly alkaline, and its effective buffer range is between pH 7.0 and 9.2. It often plays a buffer role in electrophoresis and gel experiments, and is widely used as a solvent for nucleic acids and proteins. Its derived TE, TAE, TBE and other buffer solutions are often used for DNA stability, storage and extraction.

 

HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) is weakly acidic, and its effective buffer range is between PH 6.8 and 8.2. Because it has no cytotoxicity, it can be used to prepare cell preservation solution, and also used in the research of organelles and highly volatile, pH sensitive proteins and enzymes. In addition, it can also be prepared as buffer in electrophoresis and electroporation.

 

2. Comparison in skin care

TRIS is commonly used in cosmetics to regulate PH value, which can reduce the sticky feeling of cosmetics, improve the respiratory efficiency of skin cells, and prevent pores from clogging. In addition, TRIS can be used as an odor regulator in cosmetics containing amine salts to regulate the amine taste generated by friction when applying cosmetics, so as to avoid the release of residual or persistent odor.

 

HEPES has more advantages in the duration of anti-aging effect, promoting the transdermal absorption of various effective components in cosmetics, softening the skin cutin, and promoting cell regeneration. It has the advantages of short onset time, low dosage, high penetration rate, safety to human body, non-toxic, and good physiological compatibility, and stable chemical properties.

 

HEPES produced by Hubei Xindesheng Material Co., Ltd. has obtained the submission code of cosmetics raw materials, which also proves that the quality of Desheng products is trustworthy. In addition to HEPES and TRIS, other commonly used biological buffers such as BICINE, CAPS and MOPS have been researched, developed, produced and sold. If you also need biological buffers, please click the official website of Desheng to check and purchase. I believe that Desheng's products will not let you down!