Recommended collection! Here are the types and uses of anticoagulants for blood collection

 

Anticoagulant treatment is often required for blood detection in medical laboratories, which can effectively improve the detection efficiency, and for patients, it can also reduce the waiting time and quickly know the results. However, not every anticoagulant can be used directly. The medical requirements for its performance are still very strict, for example, it needs to be used in a small amount to ensure its solubility, and it does not contain any impurities. Therefore, we will focus on the types of anticoagulants used and their preparation and use, which can also be used in the later stage.

 

1、 Heparin salt

Its anticoagulant effect is very strong, mainly by inhibiting the activity of thrombin and preventing platelet aggregation. The prepared dosage of 10 ㎎ refined heparin can anticoagulate 65-125 ml of blood, but due to the different purity and storage time of heparin products, its anticoagulant effect is also different.

 

If it is prepared into 1% solution, 0.1 ml is usually extracted and dried in a test tube during use. Each tube can anticoagulate 5-10 ml of blood. Heparin may affect the isoelectric point of protein, so it will not be used for protein determination. In addition, heparin on the market is determined by its potency. Please ask clearly when purchasing.

 

2、 Sodium citrate

It is usually prepared into 3-5% aqueous solution. Although powder can also be added, the effect may not be as good as the solution. Generally, 3-5 mg of sodium citrate solution will be added to each milliliter of blood to achieve anticoagulation.

 

Because sodium citrate has an effect on calcium in blood and interferes with the process of blood coagulation, its anticoagulant effect is slightly weaker than other anticoagulants, but it also has its advantages. It can be used in the determination of erythrocyte sedimentation rate, and the amount of solution used is approximately 5-6%.

 

3、 Potassium oxalate

As a common blood treatment agent, potassium oxalate can combine with calcium substances after fully mixing with blood to prevent blood coagulation. It is suitable for the determination of non-protein nitrogen, but not for the determination of potassium and calcium.

 

The preparation method is to weigh an appropriate amount of potassium oxalate powder, add a small amount of purified water to dissolve, and then add purified water to prepare a 10% aqueous solution. If 0.1ml of solution is added to the test tube, 5-10ml of blood can not be coagulated. If it is micro-detection, less blood can be used to prepare 2% solution. If 0.1ml is added to the test tube, 1-2ml of blood can not be coagulated.

 

4、 EDTA potassium salt, sodium salt

In addition to the use of potassium oxalate and heparin type anticoagulants, potassium EDTA is also recognized as an ideal reagent. It mainly has a certain affinity for calcium in the blood and will prevent blood coagulation after complexing. The dosage of EDTA is about 1 ml per 0.8 mg, which can anticoagulate 1 ml of blood. It can be used for a variety of anticoagulant items except for the determination of calcium, sodium and other substances.

 

As a professional supplier of blood collection anticoagulants, Desheng has produced all the products mentioned above, and has a special quality department for inspection and control. Only after all performance indicators are qualified can it be sold to customers. At present, we have received many customers' favorable comments on anticoagulants. If you also have relevant intentions, please click the website for consultation!