Two methods of serum separation

 

Serum separation is a very important link in clinical blood detection technology. The separated serum color is light yellow transparent liquid, and fibrinogen has been removed. It can provide nutrients in the human body and provide an environment suitable for cell growth. Through the analysis of separated serum and instruments and equipment, it can clearly identify whether the patient has a hidden disease, which is convenient for timely prevention or treatment. Generally, there are two methods for serum separation according to the experimental requirements. Here are some details.

 

1、 Natural coagulation separation method

1. If natural separation is required, the glass test tube with excellent material will generally be selected, and its separation effect will be higher than that of plastic material.

 

2. The outdoor temperature should not be too low, and should be kept between 25-30 degrees. Since the blood temperature inside the body is 37 degrees, after leaving the body, the room temperature is too low, which will lead to cell rupture due to heat expansion and contraction, increasing the probability of hemolysis.

 

3. If there is an incubator, it can be tilted in the incubator for about 1.5 hours, and the separation effect is better, but pay attention not to exceed 2 hours, which will also increase the probability of hemolysis. If there is no incubator, it can be tilted at room temperature overnight, so the effect is the same.

 

2、 Centrifuge method by serum separating gel

It is mainly assisted by the test tube added with serum separation gel. Because the separation gel has its own special properties, it can help to separate serum and blood cells. In addition, it can rapidly improve the efficiency of serum acquisition by using the specific gravity and using high-speed centrifuge for turnover. However, it should be noted that the first centrifugation should not exceed 3000 rpm, which is easy to cause hemolysis. In addition to plasma, if we want to ensure that blood cells are not damaged, we generally use the speed of 2000 rpm centrifugation, and the time is about 15 minutes.

 

Desheng, as the manufacturer of blood collection additives, has always been the top priority in the research and development and innovation of serum separation gel. Since its development in 2005, its quality has become stronger and stronger after several generations of improvement and continuous optimization, and has been recognized by the market and customers. At the same time, because it is an independently developed product, the process is stable and can be customized according to customer needs. If you have relevant requirements, please click the website for details!