Is the biological buffer hepes toxic to cells?

 

Various kinds of solutions are indispensable for all kinds of experiments, among which buffer is more common and is a necessity in the experiment. As one of the most popular solutions, the effect of HEPES buffer should not be underestimated. In recent years, it has been widely used in various large cell experiments and is a solvent recognized by the public. However, recently, many people have questioned the toxic effect of HEPES on cells. Is this the truth? Let me give you a detailed analysis.

 

1、 Concentration of HEPES buffer

The pKa of HEPES is 7.3 at 37 ℃, and it can be added to the culture medium at a concentration of 10mM-25mM to achieve additional buffer capacity. If the concentration is too low, it is generally impossible to control and adjust the PH value, but if the concentration is high, it will produce toxicity, so special attention should be paid to the concentration when using.

 

2、 Reasons for selecting HEPES buffer

Because metabolic behavior will occur during the whole cultivation process, resulting in changes in the PH value of the whole environment, if you want to maintain a stable environment, you need to add an appropriate amount of buffer to the culture medium. There are many buffers on the market. Why do you choose HEPES alone? In fact, this is related to its buffer range and composition. The PH range is just between 6.8 and 8.2, which is the effective range of culture. Compared with other buffer solutions, it has higher stability.

 

3、 Effect of HEPES on cells

In fact, the use of HEPES has no toxic effect on cells. It can control the range of PH value within a certain period of time, and there is no toxic side effect within the reasonable requirements of the concentration. Therefore, it will be used in various cell culture experiments to maintain the required environment.

 

In addition, the use of buffer solution will require the room temperature to reach 25 ℃, or higher temperature conditions according to the experimental requirements. HEPES has the decomposition ability and can change with the change of temperature, which can not only meet the temperature requirements of cell culture environment, but also ensure the experimental process.

 

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