Preparation method of trimethyl aminomethane hydrochloride

 

As a buffer solution commonly used in biochemical experiments, trimethylaminomethane hydrochloride has low ionic strength, good buffering effect, and can ensure the survival of protein in a suitable pH environment, so it is often used to prepare various buffers. Although it is very easy to buy trimethylaminomethane hydrochloride in the market, sometimes it is difficult to use the product due to external factors. At this time, it is necessary to fully understand its preparation process to solve the problem once and for all. Next, we will introduce its preparation process.

 

1、 Prepare materials

According to the concentration of the required solution and the amount of buffer solution, fully calculate the required amount, and prepare an appropriate amount of Tris, deionized water and hydrochloric acid.

 

2、 Preparation method

1. Firstly, the concentration and volume of Tris buffer solution should be roughly determined. For example, the concentration of brine used in Tris buffer solution may vary. If the substance to be prepared is determined, it can be calculated by the concentration of buffer solution, and then the required number of Tris moles can be obtained.

 

2. Next, multiply the molar number by the molecular weight of Tris to determine its grams. After determination, dissolve Tris powder in deionized water to reach the required volume range.

 

3. After obtaining the required volume, HCl can be mixed until the pH meter provides the PH value of the required buffer solution, and then dilute the buffer solution with water to reach the required solution volume.

 

4. After the solution is prepared, it can be stored aseptically at room temperature for several months and used as soon as possible.

 

At present, there are many manufacturers producing Tris-HCL buffer in the market, but there are not many manufacturers with excellent quality and stable process. Desheng, as an advantageous supplier of biological buffer, has continuously refined the process in recent years. The products produced have transactions in many regions of the world, and are widely praised by industry insiders. Among them, the commonly used buffer products such as Tris, Mops, Hepes, Taps, etc. are available in stock, which can provide customers with batch orders and professional after-sales services. If you are interested in Desheng products, you can click the website or call for details.