Configuration and advantages of Tris acetate

 

Tris is a necessity in experimental research and analysis. According to its own performance, it can ensure that the solution can maintain stability even if it is disturbed by the outside world. With the development of the application field, acetate can be configured on the basis of it, which is often used in nucleic acid electrophoresis buffer or buffer in other DNA-related research experiments, and sometimes used together with EDTA. As a popular reagent, let's introduce the configuration and advantages of acetate for you to understand and use.

 

1、 Tris acetate buffer configuration method

1. Prepare to weigh Tris and EDTA powder and place them in a prepared 1L beaker.

2. Add an appropriate amount of distilled water into the beaker, fully stir it until it is completely dissolved, or add it according to the volume calculated in advance.

3. Then add acetic acid into the cup, fully stir, continue to add distilled water to make it volume to the required range, and store it at room temperature.

4. Dilute when using. If the product has accumulated, it can be dissolved in a high temperature water bath.

 

2、 Advantages of Tris acetate buffer in TAE

Because it can be used as both working solution and concentrated solution in TAE, each has its own advantages. The advantage of working solution is that it is easy to operate, which can reduce the experimental operation steps, and it can reduce the risk of infection when operating under sterile condition. However, the use cost of concentrated solution in TAE is not high, and its scope of application is very wide. If sterile operation is not required, it can also be used in experimental studies of various concentrations. In addition, the high concentration makes it more stable to store.

 

Although Tris acetic acid buffer will be widely used in TAE, TBE buffer will also be used sometimes. Generally, the purchaser will also measure which buffer is more suitable for the experimental requirements. Desheng, as a manufacturer specializing in the production of biological buffer, can provide raw materials such as Tris, Tris hydrochloride, Tris acetate, etc. The production process is simple and stable, and the purity is up to 99%, which basically does not interfere with the experiment. In recent years, buffer products have been sold in large quantities, and nearly 1000 customers have cooperated with them. The feedback received is favorable. If you have relevant requirements, please call to inquire details!