Characteristics of PIPES buffer and PB buffer

 

In recent years, Good's buffer has attracted great attention and has been frequently used in various biological experimental studies, while PIPES buffer and PB buffer are two kinds of buffer under Good's buffer, which have also become "sweet cakes".

 

Phosphate buffer solution was widely used in biological experiments in the early stage. With the advancement of experimental technology, the requirements of various experiments are getting higher and higher. Phosphate buffer solution can no longer meet the extensive use needs, so buffer solutions such as PIPES, PB and Tris have been developed. Next, we will focus on the characteristics of PIPES buffer and PB buffer for your convenience.

 

Characteristics of PB buffer

As a ternary acid, PB buffer solution can undergo secondary dissociation in the solution, and its pKa value also has two, so the pH range is very large, and the potassium salt using phosphate is better than the sodium salt in the preparation process, because the sodium salt is difficult to dissolve at low temperature, while the potassium salt is easy to dissolve. Moreover, because its applicable PH value range is relatively wide, and it is less affected by temperature, even if it is diluted, PH will not change greatly.

 

Characteristics of PIPES buffer

Although PB buffer has good buffering performance, in the solution system with metal ions, phosphoric acid system will associate with common Ca, Mg and heavy metal ions to form precipitation. However, PIPES buffer will not precipitate in the solution system containing metal ions, and is suitable for the detection system involving multiple proteins and enzymes.

 

In addition, although the pH value of PIPES can not be as wide as that of PB buffer solution and can cover the pH range of 2-12, for biological experiments, many enzymes and proteins adapt to the pH value of about 7, and the pH value of PIPES buffer is just about 7, which meets the requirements and is better in performance.

 

Desheng is an advantageous manufacturer of biological buffer, including not only PIPES buffer solution, PB buffer solution, but also Tris, Bicine, Caps and other buffer solutions, with a purity of more than 99% and stable process. At present, there are goods in stock in the warehouse, which can be supplied in batches and delivered in time to ensure the customer's demand for goods. For product quality problems, professional after-sales team service is provided to ensure that there is no worry about returning and exchanging goods and the use is safe. If you have relevant requirements, please click the website for details!