Introduction of biological buffer CAPSO

 

Biological buffer plays an important role in biochemical analysis, in vitro diagnosis and other fields, and CAPSO, as one of the types of buffer, is gradually applied in a wide range of fields. As an amphoteric ion buffer, its appearance is white powder with good solubility in water. Compared with other insoluble buffers, CAPSO is more convenient to use. Let's introduce it to you in detail.

 

1、 Preparation of CAPSO buffer

1. Prepare CAPSO sodium salt powder, distilled water (dH2O), stirring rod and beaker in the required proportion for the experiment.

2. Pour CAPSO sodium salt and corresponding proportion of distilled water into a beaker, and stir with a stirring rod until CAPSO sodium salt powder is dissolved.

3. Then it is necessary to adjust to the required PH value, and then pour distilled water (dH2O) to the required volume.

4. The finished CAPSO solution can be obtained by filtration, disinfection and sterilization.

 

2、 Application of CAPSO buffer

1. It is often used in enzyme-catalyzed reactions and the preparation of some detection reagents, or in immunoblotting and precipitation experiments.

2. It can be prepared into a lysate for extracting membrane protein. CAPSO can be used as a buffer to prevent major changes in pH, keep ionic strength within a reasonable range, and prevent protein from combining with other ions to form insoluble products.

3. It can provide a good storage condition for various experimental solutions, which is conducive to stable and effective storage and avoid adverse reactions.

4. It can be used in medical diagnostic kits, and has great significance in the generation and treatment of liver diseases, myocarditis and other diseases.

 

3、 Use of CAPSO buffer

1. As the produced CAPSO is in powder form, pay attention to avoid moisture during use, store it in a closed manner at normal temperature, and seal it immediately after use. If it is a prepared solution, it needs to be stored at a low temperature of 4 ℃.

2. In order to make CAPSO have a better buffering effect, it is recommended to use it as soon as it is available to avoid long time shelving, which will easily lead to deterioration and affect the test process.

3. During the experiment, it is necessary to do a good job of protection and wear protective equipment to avoid direct contact with the skin. If you accidentally touch the skin, rinse it with flowing water in time.

 

Desheng is a direct supplier of biological buffer. Its own research, development, production and sales not only ensure the purity, but also control the process and raw materials. Even if there is a problem, it can also provide solutions at the first time and provide one-stop service for customers. If you have any intention, please call for details!