Manufacturing process of high purity CAPS buffer

 

CAPS plays an important role in various analytical studies, but it is more widely known that it can adjust PH value. However, with the improvement of its performance, it is also widely used in other fields, such as biochemical analysis, nucleic acid extraction or coating. All applications in these fields are inseparable from its manufacturing process. If the process is unstable, it is easy to lead to poor purity and performance, and there are many problems in use. Now let's give you a detailed analysis of its manufacturing process.

 

The production and synthesis process of CAPS plays a decisive role in the quality of CAPS finished products. At present, the production processes mainly include the following:

1. Prepare raw material ethanol, cyclohexylamine and propionate lactone, first dissolve cyclohexylamine in ethanol, then slowly add propionate lactone, pay attention to the feeding speed and ambient temperature, and keep it at 50 ℃. After the completion of feeding, continue to react for about 4 hours to get the crude product, then dissolve it in hot water, add ethanol, and after cooling and crystallization, CAPS will be produced, and the crystallization will be filtered and dried to get the finished product. Although this method is simple, the finished product is easy to have suspended matter after dissolution, and the absorbance is poor, and the yield of the finished product is low and the quality is poor.

 

2. To prepare dimethylformamide, propionolactone and cyclohexylamine, first dissolve the propionolactone, then add cyclohexylamine at the preset speed, pay attention to keep the ambient temperature at about 30 ℃, keep the reaction for several hours after feeding, filter and dry to get the crude product. After that, the crude product is dissolved in hot water, added with ethanol, precipitated after cooling, filtered and dried. The quality of this product is relatively high.

 

3. Prepare the raw materials cyclohexylamine and propionolactone, dissolve them in different organic solvents respectively, and then pump them into the microchannel reactor for full reaction. The obtained solution is fully cooled and crystallized, and the crude product is obtained after being filtered by nitrogen pressure. The process of this method is simple, stable and controllable, and the product yield and output are improved, but the required equipment will be more advanced.

 

The above three methods are CAPS manufacturing processes, each with its advantages and disadvantages, but most manufacturers use the third method because of its high efficiency and more stable process. Desheng is a professional manufacturer of CAPS buffer solution, with advanced technology, high quality raw materials and no impurities, so the purity of the finished product can reach more than 99%, and it is more reliable to use in various fields. If you have relevant requirements, please click the website for details!