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What effect does anticoagulant EDTA potassium have on blood routine test?

Release time: 2022-12-28

What effect does anticoagulant EDTA potassium have on blood routine test?
 
As a commonly used reagent in medical blood testing, there are many types of extracorporeal anticoagulants. Among them, potassium dichromate in EDTA potassium salt is recommended by the International Committee, and as an ideal anticoagulant in blood samples, it has no significant difference in test results within 1-4 hours after blood samples are collected. Although it is a commonly used reagent in blood sampling, many people do not know how it affects the routine blood sample test. Here is a detailed introduction.
 
1、 Affect blood cell volume
 
Because almost all EDTA salts can lead to cell shrinkage, it will affect the micro specific volume of cells after centrifugation. In addition, the solubility of potassium salt is greater than that of sodium salt, so we generally choose potassium EDTA, and use less sodium EDTA. At the same time, because the PH of EDTA dipotassium is lower than that of EDTA tripotassium, and the cells swell, it can compensate for the cell shrinkage caused by osmotic pressure. In this way, when using the cell analyzer, it will be found that the specific volume of cells using EDTA dipotassium anticoagulant is more satisfactory.
 
2、 Affect platelet
 
EDTA anticoagulant was added in the detection process. It was observed that the platelets would change from circular to spherical, which would affect the determination of platelet volume. In addition, the adhesion and aggregation changes of platelets will gradually intensify with the extension of the storage time of blood samples, which will affect the number of platelet counts. If it is really necessary to determine the platelet count, the method of sodium citrate anticoagulant can be used.
 
3、 Affect white blood cells
 
EDTA will have a variation effect on the stability of leukocytes, and in different analysis fields, the variation generated by leukocytes will add up, making lymphocytes more stable and monocytes affected.
 
4、 Affect neutrophils
 
The concentration of EDTA potassium salt will cause obvious changes in the morphology of neutrophils. Therefore, attention should be paid to the addition concentration of EDTA anticoagulant during blood collection. If the concentration is too high, it will affect the morphology of neutrophils, mainly manifested as loss of lobulation, unclear cytoplasmic boundaries, and left shift of nucleus.
 
Desheng is a professional manufacturer of anticoagulants. Its EDTA potassium salt has a purity of more than 99%. It is popular in the market as an additive in the test tube. It has been constantly consulted and purchased. With excellent quality and sufficient inventory, it is the unanimous choice of the public. If you have relevant needs, please click the website or call for details!