Effect of anticoagulant on biochemical detection

 

At present, there are three kinds of closed blood collection tubes and their corresponding anticoagulants used in China: blue sodium citrate anticoagulant tube, purple EDTA potassium salt anticoagulant tube, and green heparin anticoagulant tube. These three colors are different, and their uses are also different. Generally, purple is used for routine blood tests, while blue is mainly used to detect blood coagulation, and green is used for infectious immune diseases. So what is the specific impact of the anticoagulants in the three test tubes on the biochemical test items? Here's a detailed introduction.

 

1、 EDTA potassium salt anticoagulant tube

 

This is an anticoagulant widely used in clinical practice. It can well maintain the morphology of blood cells and platelets. The impact on biochemical detection is that it can interfere with the detection of potassium ions contained in blood in test tubes. At the same time, because it can chelate with calcium ions, it can reduce the concentration and affect the detection of calcium ions.

 

2、 Sodium citrate anticoagulant tube

 

The effect of sodium citrate is mainly because it can complex with calcium ions to form a chelate, thus preventing the formation of fibrin at the source. Because it contains sodium, it will affect the detection of sodium ions. In addition, it will also affect the activity of enzymes.

3、 Heparin anticoagulant tube

 

This anticoagulant test tube is different from the other two anticoagulant test tubes, mainly relying on heparin to produce effects. It can enhance the activity of antithrombin 3, inactivate serine protease, and prevent the formation of thrombin. Although it has a small impact on biochemical detection, it is not conducive to the detection of total bilirubin, because heparin and surfactant p-aminobenzenesulfonic acid will produce turbidity, which will affect the detection results of total bilirubin.

 

The impacts mentioned above are mainly for items that cannot be detected and are added to the test tube as reagents. As long as the limited items are not detected, there will be no other impacts. Desheng is a professional supplier of blood collection tube additives. Before selling, the company will strictly monitor the product quality and analyze data indicators. Only after all indicators are tested to be qualified, can it be sold to customers, and strictly prevent the sale of false or low-quality products. In order to satisfy customers, the company has set up a special after-sales service department to ensure that customer problems can be solved at the first time, to provide customers with goods risk services, and to return and replace goods without worry. If you have relevant needs, please click the website or call for details!