A Preparation Method of MES 2-Morpholineethanesulfonic Acid

 

MES is one of the good ′ s buffer solutions, which is widely used. MES is a zwitterionic N-substituted amino sulfonic acid with morpholine ring. This component does not form complexes with most metals used in environmental and biological research. It is easily soluble in water but extremely difficult to dissolve in lipid, so it cannot penetrate the membrane, so it has advantages in the application of cell culture medium.

 

As a biological buffer, MES is often used for the isolation and culture of protoplasts of various plants, for example, to promote the growth of Arabidopsis thaliana by affecting the pH change of the culture medium; MES is also used as eluent buffer component, which can be obtained from α Plasma was eluted on 2-macroglobulin affinity column; In the process of antibody binding to particles, this component is used as an activation buffer; In the process of creating microfluidic integrated surface plasmon resonance (SPR) platform for pathogen detection, MES can be used for microchannel functionalization, etc.

 

According to the MES sold on the market, the purity levels are different, which may be due to the different production methods used by the manufacturers. Next, we will introduce a preparation method of MES. Let's learn about it!

 

Preparation method of MES

1. The organic solution of morpholine and sodium chloroethanesulfonate is reduced to pH 9 under the condition of heating and reflux;

2. Slowly add sodium methoxide into the above solution to control the pH value of the reaction system;

3. After the reaction, methanol solution of 2-morpholineethanesulfonic acid is obtained;

4. Add hydrochloric acid solution to the solution and adjust the pH value to 5;

5. Then, sodium chloride is removed through thermal filtration to obtain 2-morpholineethanesulfonic acid solution for freezing crystallization;

6. Finally, the crystallized crystal is centrifuged to obtain the finished product of 2-morpholineethanesulfonic acid.

 

This method is not only cheap, but also the yield of 2-morpholineethanesulfonic acid is more than 90% and the purity is more than 99%. It greatly improves the production efficiency and product quality. Desheng is a manufacturer of biological buffers. At present, the buffers produced are of high purity and wide range of applications. A complete range of products can meet the one-stop shopping, if you need to buy, please call at any time to inquire and order!