What is the ratio of anticoagulant sodium citrate to blood in coagulation project?

 

When we go to the hospital for physical examination, we can find that the blood coagulation test item is one of the routine items in the hospital. It can not only play a role in screening blood coagulation diseases, but also be used to evaluate the effect of patients' anticoagulants, and become an important indicator of auxiliary diagnosis.

 

However, there are many factors affecting the coagulation analysis, such as clots, hemolysis and the addition of anticoagulants. Therefore, operators will pay special attention to these aspects during the inspection. What is the proportion of anticoagulant addition to blood in the inspection?

 

At present, the anticoagulant used in the blood coagulation test project is sodium citrate. Generally, the ratio of sodium citrate to blood is 1:9. A blue anticoagulant tube with a specification of 3ml is used. The tube contains approximately 0.3ml of anticoagulant. The corresponding blood collection volume is 2.7ml. If the whole blood ratio is based on HCT and plasma, the ratio is 1:5.

 

In the coagulation process, both PT and APTT reactions require calcium ions to participate. If the proportion of anticoagulant and plasma is misadjusted, it will have a significant impact on the test results. If the anemia is too serious, the plasma becomes more. If the anticoagulant remains unchanged, the blood volume needs to be adjusted to make the ratio of anticoagulant to plasma 1:5, which meets the experimental requirements. Otherwise, if it is not adjusted, there is too much plasma, and calcium ions cannot combine with sodium citrate, leading to the early start of the coagulation process and the elimination of coagulation factors, which will lead to "false" results.

 

In addition, there is another situation that the proportion of plasma decreases when the patient is dehydrated due to continuous diarrhea. If the content of anticoagulant is too high during testing, the limited calcium ions in the reaction process will be completely chelated by the anticoagulant, which will also result in "false" results. Therefore, the dosage of anticoagulant needs to be controlled.

 

Although conventional standards and specifications emphasize the need to adjust the dosage of anticoagulant, in the actual operation process, the blood collection volume should be adjusted. Therefore, in clinical practice, medical staff will pay special attention to the blood collection volume in the blue test tube with sodium citrate at 1.8 mL, rather than the usual 2.7 mL. Sometimes, the proportion of blood collection will be adjusted again according to the patient's condition, so it is not impossible to adjust it. In the experiment, be careful not to be rigid.

 

Desheng is a manufacturer of additives for blood collection vessels. All products are supported by the medical industry. Both anticoagulants and anticoagulants are AR analytical pure grade, with stable performance and no impact on any experiment. If you have related needs, please call us!