Selection requirements for the use of biological buffers in experiments

 

As an important experimental solution, buffer solution often appears in various biological experiment operations. Its main role is to balance the PH value of the reaction system. As one of the preferred solutions, there are also many conditions and restrictions when choosing to use it in experiments. So next, let's talk about the selection requirements of buffer solution in the experiment.

 

1、 Selection of pH value of biological buffer

 

The buffer range in the experiment must conform to the pH value of the reaction system to ensure the accuracy of the experimental results and avoid interference during the operation. The pH value of the buffer range usually depends on the ionization number pKa, but the pKa value is related to the salt or acid concentration, so it is often calculated according to the formula. Theoretically, the buffer range is between 9.4-11.7, but in order to ensure accuracy in actual experiments, buffer solutions with pH values between 9.7-11.7 are usually used, such as CAPS buffer solution. Sometimes, other ranges of pH values may be used according to the purpose of the experiment, but they all need to be selected, and the same range of pH values cannot be used completely.

 

2、 Ionization balance constant and buffer range requirements

 

In many measurement experiments, the PH value of the reaction solution is required to be stable within a certain range to ensure that the experiment process does not change. By adding a certain amount of buffer solution, neutralization is achieved, so that the buffer range is controlled within the required range. In addition, sometimes the titration curve of the same buffer solution with different ratios is required when the potentiometric titration method is used for determination. In this case, there are also requirements for the ionization equilibrium constant, which requires special attention.

 

3、 Restrictions on the use of biological buffers

 

In general, if the buffer range is reasonable and in line with the reaction system, it is necessary to consider the limiting conditions of the reaction system. For example, PBS buffer solution will complexe metal ions such as calcium, enzymes, etc., while the activity of enzymes or proteins in some reaction systems is affected by metal ions, so this buffer solution cannot be used. In addition, there are some other types of buffer that need to be used according to the purpose of experimental determination, and not all biological experiments can use one buffer.

 

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