Precautions for using PIPES buffer

 

Piperazine-1,4-diethanesulfonic acid, the full name of PIPES, is a kind of zwitterionic biological buffer and also one of GOOD'S buffers. It has excellent characteristics such as high water solubility, bottom cell membrane permeability, stable acid-base dissociation, low metal chelating ability, high chemical stability, and low absorption spectrum in ultraviolet and visible light regions.

 

As a very widely used buffer, PIPES can minimize lipid damage and experimental artifacts caused by pentylenediol fixation in phytochemistry. Because the pka of PIPES is very close to the physiological PH value, it is very suitable for cell culture in vitro. However, PIPES can form free radicals, so it is not suitable for redox reaction.

 

PIPES buffer

 

What should we pay attention to when using PIPES?

 

1. In order to ensure the accuracy of the experiment, it is necessary to first clean the used container with clean water for more than three times, then ensure that the solution used is not pure water, and test the blank value of the solution required for the experiment in advance.

2. As PIPES is a solution sensitive to light and easy to decompose, it is better to store it in a special brown reagent bottle with a stopper to minimize the impact of the outside on PIPES buffer.

 

3. After the PIPES buffer solution is configured, the configuration time, concentration, specification, name and other information should be noted in time to facilitate the use of subsequent experiments. And it is better to use it whenever necessary to ensure the accuracy of the experimental results.

 

4. When using PIPES, it is necessary to do a good job of protection to avoid certain damage caused by touching the skin.

 

5. It is necessary to prepare the buffer with the most appropriate concentration, because when screening cells in the experiment, the buffer with too high concentration will kill all cells, and the buffer with too low concentration will not play the role of screening. Both conditions will interfere with the final experimental results.

 

Hubei Xindesheng Material Science and Technology Co., Ltd. specializes in the production of GOOD'S buffer. After 17 years of research, it has been able to produce more than ten kinds of most common biological buffer solutions on the market, such as PIPES, TRIS, BICINE, CAPS, MOPS, and has also been recognized by customers. If you also need to buy a biological buffer, you can click Desheng's official website for consultation, and Desheng will give you a satisfactory answer!