EPPS (HEPPS) series
EPPS (HEPPS), physiological buffer, effective pH buffer range (7.3-8.7), pKa 8.0 (25°C). Ultra-thin isopoint focusing gel is used as a separating agent to improve the resolution of glucose phosphate mutase.
HEPPSO sodium, zwitterion hydrogen buffer, isopoint focusing electrophoresis (positive EF) separation of proteins. There are many ways to prepare HEPPSO buffer. Or dissolve HEPPS with water, then adjust to the required pH with NaOH or KOH. Or mix HEPPSO and HEPPSO sodium salt in equimolar concentration, and then use one of the reagents to adjust to the required pH.
1. Tris ethanesulfonic acid (TES)
Application description: pKa7.4 (physiological pH), is a very widely used biological buffer reagent. TES is suitable for various cell culture systems that require divalent metal ions. However, many other buffers (such as citrate or phosphate) can cause chelation or precipitation reactions, making it unable to meet this requirement.
Bis-Tris, Chinese name Bis(2-hydroxyethyl)amino(trihydroxymethyl)methane, CAS NO.6976-37-0, an inert zwitterionic buffer, its dissociation constant changes with temperature Only minor changes occur. It is suitable for the separation of hemoglobin, and can protect the stability of hemoglobin during freeze-drying storage. It can be used as an effective buffer for muscle enzyme activity analysis. Used as a catholyte in 2-D gel electrophoresis isoelectric focusing electrophoresis (IEF).
2. Tricine
Application description: One of ZUI's wide range of buffers, commonly used as electrophoresis buffer, and used to resuspend cell pellets. Tricine has the characteristics of low negative charge and high ionic strength, which is very suitable for electrophoresis separation of low molecular weight proteins of 1-100 kDa. One of the buffer components used to separate low molecular weight peptides, an effective hydroxyl radical scavenger.
3. PIPES, POPSO
PIPES, pKa is close to physiological pH, suitable for cell culture operations. The use of glutaraldehyde histological buffer in plant and animal tissue research can reduce lipid loss. POPSO: used to store DNA samples, during the amonially labeling process to avoid cross-reaction with the first amine group.
It can be seen from the above introduction that because of the uniqueness of different types of biological buffers, there is still a big gap in application. As a professional manufacturer of biological buffers, Desheng has many years of research and development experience and more professional As support, the technical team has the ability to provide customers with products with more favorable prices and more guaranteed quality. If you happen to have procurement needs in this regard, please enter the official website to contact professional customer service for ordering consultation.