Relationship between HEPES and HEPES Na

 

HEPES and HEPES Na are very similar in terms of names, so it is easy to link them together. First of all, from the application point of view, both products belong to biological buffers, and have similar uses in biochemical experiments, daily chemicals and other fields. So what's the relationship between the two? Let's take a closer look.

 

HEPES and HEPES Na are actually conjugated acids and bases. The difference between them is that the pH value after dissolution will be different. According to the synthetic method of HEPES Na recorded in the literature, HEPES is a component in the synthetic raw materials. It is a widely used preparation method of HEPES Na to produce HEPES Na through the reaction of HEPES and NaOH. Using this method to produce HEPES Na, not only the process is simple, but also the yield is very high.

 

The detailed steps are as follows:

1. When HEPES and NaOH solution with a purity of about 95% are reacted under nitrogen protection, the reaction time shall not exceed 1 hour. (reaction temperature is 20 to 100 ℃);

 

2. After the reaction, the product is decolorized, filtered, concentrated, dehydrated, crystallized, washed, etc;

 

3. After the above steps are completed, HEPES Na powder is obtained after drying.

 

It can be seen from the above that HEPES Na is a derivative buffer of HEPES, and a positive solution is also obtained for why they are so similar. I believe that the next time I see these two products, I will never be confused. HEPES is used more frequently than HEPES Na, especially in cell culture. As a cosmetic additive, it can also play the role of active agent, peeling agent, penetration enhancer, etc.

 

Desheng Biochemical has many years of experience in the R&D and production of biological buffers, and has been actively promoting the promotion and use of biological buffers in more fields. At present, the biological buffer products produced by Desheng have been widely used in in vitro diagnosis, chemical raw materials, biological cosmetics, paint and other industries. If you need to buy biological buffers, you can go to the website of Desheng for more product information.