Anticoagulants and coagulants, which is more useful?

Both anticoagulants and coagulants are essential additives in vacuum blood collection tubes for blood testing. Depending on the test items, different coagulants and anticoagulants will be added. Also as a blood collection tube additive, what are the differences in the role and use of coagulants and anticoagulants? Which will be more useful?

1. The role and use of coagulant

When the coagulant is added to the vacuum blood collection tube, it can activate fibrinase, so that soluble fibrin becomes insoluble fibrin aggregate, which can accelerate blood coagulation and shorten the test time. It is usually used together with serum separation gel, mainly for serum biochemistry (liver function, kidney function, myocardial enzymes, amylase, etc.), electrolytes (serum potassium, sodium, chloride, calcium, phosphorus, etc.), thyroid function, AIDS testing, tumor markers, serum immunity, etc.

2. The role and use of anticoagulants

1. Sodium heparin and lithium heparin have the effect of strengthening the antithrombin inactivation of serine protein, thus preventing the formation of thrombin and platelet aggregation, with minimal interference to blood components, without affecting the volume of red blood cells or causing hemolysis. It is suitable red blood cell osmotic fragility test, plasma osmotic volume, red blood cell stack, erythrocyte sedimentation rate and general biochemical determination. It is worth noting that excessive heparin will cause the aggregation of white blood cells, which cannot be used for counting white blood cells, nor is it suitable for white blood cell classification.

2. Dipotassium, tripotassium and disodium edetate can effectively chelate calcium ions in the blood to prevent blood coagulation.They have little effect on the coagulation of blood cells and the shape of blood cells. And they can inhibit the aggregation of platelets in general blood tests. However, EDTA can inhibit or interfere with the polymerization of fibrin monomers during the formation of fibrin clots, so it is not suitable for blood coagulation and platelet function tests, nor is it suitable for the detection of calcium, potassium, sodium and nitrogen-containing substances.

3. Trisodium citrate acts on calcium ion chelation in blood samples to achieve its anticoagulant effect when used in coagulation tests. And because trisodium citrate has little effect and toxicity on MPV and other coagulation factors, it is often used in platelet function analysis and blood preservation. When the added trisodium citrate reaches a certain concentration, it can be used for erythrocyte sedimentation test.

According to the above analysis, it can be seen that coagulants and anticoagulants have their own uses, and both have irreplaceable roles in blood tests. As a high-quality manufacturer that can independently produce a complete set of blood collection tube additives, Desheng can provide you with a one-stop service experience. If you have relevant needs, please click on the official website to inquire!