Common Buffer Solutions in Biochemical Experiments

As the name suggests, buffer solution actually means that it can play a buffer role. It is a solvent composed of acid base conjugated pairs. When a certain acid or base is added in the experiment, it can stabilize the PH value within a reasonable range without changing. Because of its good buffer capacity, it is very popular in major biochemical experiments. So, what are the commonly used buffers? Now let's introduce it to you.

1、 Phosphate buffer

Phosphate buffer solution is widely used. Due to its secondary dissociation effect, its pH range is wide, and it can be used to prepare acid-base and neutral buffer solutions with different pH values. For example, potassium dihydrogen phosphate can be directly used to prepare acidic buffer solutions, with the pH range less than 5. Disodium hydrogen phosphate can be used to prepare alkaline buffer solutions, with the pH range greater than 5. At the same time, phosphate buffer solutions are basically not affected by temperature.

2、 Tris buffer

Tris is widely used as phosphate buffer, but it is a weak alkaline solution, usually used in the "neutral" range. In addition, it can also derive a variety of biochemical buffers, such as Tris hcl. Due to its own properties, it does not precipitate with the metal ions contained in the solution, does not affect the biochemical experiment process, and improves the efficiency.

3、 Glycine buffer

Glycine buffer is mainly used in cell experiments. The pH range is relatively wide, between 2-11, which is usually conducive to providing a more natural and comfortable living environment for cell components and various extracts.

The above three kinds of buffer solutions are commonly used in biochemical experiments. There are many types of buffer solutions. When you use them, you need to compare them according to your own needs. You can roughly check the water solubility and stability of the buffer solution, as well as the buffer capacity and compatibility within the required PH value range, so as to select the buffer solution that meets the experimental requirements.

Desheng Biochemical Co., Ltd. is an advantageous manufacturer specializing in biological buffer solutions. Its R&D and production have lasted for decades. At present, the biological buffer products produced include TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PEP, etc., with a complete range, which can be customized by customers. The factory has direct sales, favorable price, large warehouse, stable supply, guaranteed continuous supply, high production efficiency, fast delivery efficiency, and instant delivery. If you have relevant needs, please call us for consultation!