What is the purpose and function of adding buffer in biochemical experiments?

Biological buffer is a solution that can keep the pH value unaffected by pH. Many cells can only live in a small pH range, and a buffer system is required to maintain the entire process undisturbed.

Especially in biochemical experiments, biological buffers are very important, because various substances in the experiment are very easy to change the pH, which directly affects the overall work process.

For example, in enzyme activity experiments, sometimes due to large changes in pH, the enzyme activity will decrease or even be inactivated, resulting in the inability of the experiment to proceed. Therefore, the main purpose of adding buffers in biochemical experiments is to maintain pH stability.

I.Why can the buffer solution maintain the stability of the pH value?

In fact, this is mainly due to the composition and performance of the buffer solution itself. Its composition contains acids, salts or alkalis, so it can offset the effects of adding acidic or alkaline solutions from the outside to a certain extent.

II.What are the functions of preparing buffer solutions?

1. Buffer solution is a solution that is often used in analysis and detection experiments. In experiments, the pH value of the solution is usually required to be kept within a specified range to ensure the color development or discoloration of the indicator. These can be achieved by adding a certain amount of buffer solution. 

2. Buffer solutions also play an important role in medical testing, mainly because buffer solutions can maintain the normal range of blood pH in the body. Carbonic acid-sodium bicarbonate is the main buffer pair in the blood, which is closely related to lung respiration and renal function. Excretion is closely related. The CO2 produced by the body's normal metabolism will enter the blood and combine with water to form carbonic acid, which in turn forms a conjugated acid-base pair with bicarbonate ions in the plasma, so the buffer solution has a role that cannot be ignored in medicine.

There are many types of biological buffers, and the appropriate system needs to be selected according to the actual application. Desheng Biochemical is an advantageous supplier of biological buffers, specializing in providing various buffers, such as: Tris, Caps, Mops, Bicine, etc. Excellent performance, high purity, long-term customer consultation, bulk purchase, standard pre-sales and after-sales service, if there are product problems, professionals will provide comprehensive answers and guidance. If you have related needs, welcome to call us to buy!