Acridine ester chemiluminescence analysis CLIA is a very promising immunoassay method currently in the market. The tubular chemiluminescence magnetic bead method usually uses streptavidin and biotin. Here is an introduction to the role of streptavidin in CLIA .
The chemiluminescence of acridine esters is different from that of luminol. Luminol is a luminous type and can emit light for a long time, while acridine esters are flashing, with a short luminescence time and a rapid luminescence, usually only a few seconds. In the acridine ester system, the emission light intensity reaches the maximum after adding the activator hydrogen peroxide 0.4s under alkaline conditions, the half-life is 0.9s, and the luminescence basically ends within 2s, which can realize rapid detection. The combination of acridinium ester and streptavidin can make it have better performance.
The amplification effect of streptavidin in the luminescence of acridinium ester:
Streptavidin is a protein secreted by Streptomyces bacteria. It can specifically bind to biotin, and the binding force between them is the strongest non-covalent binding force known so far. Because of this feature, the streptavidin-biotin reaction system has been widely used in the field of purification and detection. Streptavidin exists in the form of homotetramer, and each mole of tetramer molecule can bind 4 moles of biotin molecule, so it has a signal amplification effect in the field of immunodetection.
Acridine ester chemiluminescence, streptavidin magnetic beads-biotin amplification reaction system includes: acridinium ester-labeled antibody or antigen, biotin-labeled antibody or antigen, streptavidin coated with carboxyl group or Tosyl (P-toluenesulfonyl) functional group magnetic beads; the particle size of the magnetic beads is 0.1-5μm. Since biotin and streptavidin are combined in a ratio of 4:1, the acridinium ester-biotin-antigen antibody complex is also combined with streptavidin in a 4-fold ratio, so that the luminescence signal is also expanded 4 times, the detection sensitivity is higher.
The sensitivity and specificity of the chemiluminescence immunoassay technology of acridinium esters are very high. After introducing the specifically bound streptavidin-biotin system, the detection signal is amplified and the CLIA of acridinium esters has a better Good practicality. Desheng is committed to the research of CLIA and magnetic beads, and a variety of acridinium ester reagents have been well received by the market.