The Chinese name of MOPS buffer is 3-(N-morpholinyl)propanesulfonic acid, which belongs to zwitterionic buffer. It is white powder with high hydrophilicity and excellent water solubility (1000g/L at 20℃) . The appearance of the dissolved MOPS in its aqueous solution is also colorless. The pKa value of MOPS at 25°C is 7.14, and the effective pH range is 6.5-7.9. Therefore, MOPS is often used in molecular biology and biochemical research.
The main application areas of MOPS as a buffer are as follows:
(1) In vitro diagnosis: biochemical kits, DNA/RNA extraction kits and PCR kits.
(2) Protein/nucleic acid separation and purification: separation in nucleic acid and protein electrophoresis, RNA1 denaturing gel electrophoresis, and protein purification in chromatography.
(3) Cell culture medium: the buffer system of the culture medium.
(4) Biosynthesis: the buffer used in biosynthesis.
(5) Measure absorption in ultraviolet/visible light spectrophotometry and use cyclic voltammetry to study redox characteristics.
(6) When controlling the pH of the culture medium to 5, including cell culture medium for yeast, bacteria and mammalian cells.
Matters needing attention when using MOPS buffer:
When mops buffer is used for mammalian cell culture, the concentration of MOPS cannot be higher than 20mm;
There is almost no complexation between MOPS and metal, but once complexation occurs, interference may occur;
MOPS can affect the thickness and barrier properties of the rat endothelial surface layer;
In the presence of glucose, MOPS will be partially degraded during autoclaving.
MOPS interacts with DNA and forms a complex;
MOPS can change the interaction of lipids;
Hubei Xindesheng Materials specializes in the research and development and production of biological buffers. It has been established for decades. The biological buffer products currently produced include a series of biological buffers such as MOPS, TRIS, HEPES, TAPS, CAPS, BICINE, EPPS, PEP, etc. Available for customers to choose. The price is favorable, the factory sends directly, so you can easily buy affordable and high-quality products. High production efficiency, fast delivery efficiency, sufficient supply, immediate delivery. Please feel free to contact us if necessary!