Tris-HCL and HEPES are both buffers, what is the difference in their advantages?

In biochemical experiments, biological buffers are often used.There are too many types to choose. Maybe the buffers selected at that time are very consistent on the surface, but there are certain differences in actual use. This directly leads to the inability of the experiment to proceed, and may also waste a lot of money and time. At this time, it is particularly important to purchase suitable and cost-effective buffers. So what are the advantages and differences between different buffers? Follow Desheng to figure out Tris-HCL and HEPES buffers in detail.

Tris-HCl buffer

In fact, Tris buffer has been widely used in the detection and analysis of biochemical experiments. It is a weak alkaline buffer, and the effective pH is neutral, so the pH of Tris-HCl buffer is between 7.5 and 8.5.

Advantages of Tris-HCl buffers

Tris-HCl has stable performance, good compatibility, and won’t produce precipitation with calcium ions, magnesium ions.But phosphate with calcium and magnesium ions will produce precipitation. And because the ionic strength in Tris-HCl buffer is weak, that determines the inactivation of enzymes, so it’s very important for enzyme determination.

HEPES buffer

The differences between HEPES and Tris-HCl buffer is that the HEPES buffer is not an alkaline or acidic buffer, but a non-ionic amphoteric buffer. Diagnostic kits, and DNA/RNA extraction kits.

Advantages of HEPES buffers

HEPES has good stability in water and its pKa value is between 6-8. The solubility is good and without interference,as well as very low absorption in visible light and ultraviolet light. It is resistant to chemical and enzymatic hydrolysis, and does not interact with metals or precipitation of ions occurs.The ion concentration, solution composition and temperature have little effect on dissociation.

Both HEPES and Tris-HCl produced by Desheng are in powder form. It is recommended to use them immediately after they are prepared into solutions with better effect. They are usually stored in a dry indoor environment without long-term exposure to sunlight and air. .

Hubei New Desheng is a professional manufacturer of biological buffers. It has a complete set of standards in both product sales and service. It puts customers' rights and interests first and provides effective solutions to customers' problems. Now the company has formed a high-quality reputation, the products are exported to many countries around the world, and a long-term contract has been signed with it. If you have any needs in this regard, please click on the official website to consult it.