Water solubility of buffers, chromogenic reagents and luminescent reagents

Many in vitro diagnostic reagents are used in a solution environment, and water is the preferred solvent, so the solubility of diagnostic reagents in water becomes the key. At present, there are three main types of in vitro diagnostic reagents produced by Desheng, which are buffering reagents, chromogenic reagents and luminescent reagents. How about the water solubility of these three reagents? Below, I will introduce it to you in detail.

The water solubility of buffers

Commonly used types of biological buffers are Tris, Bicine, MOPS, and phosphate. Most of these substances are amphoteric acids, bases or salts, and they have good water solubility. Among them, the buffer PIPES, although its water solubility is not good, for neutral solutions, it can be dissolved in an alkaline solution, or the form of PIPES sodium salt can be used, so that it can have good water solubility.

2. Water solubility of color reagents

The chromogenic reagents have been used in the early days, and there are many kinds. Desheng Biochemical focuses on research and development and production of new Trinder's reagents, which are chromogen substrates for enzymatic reactions, including TOOS, TOPS, MAOS, ADOS, etc. The water solubility of traditional Trinder's reagents or aniline derivatives is relatively unsatisfactory, but after the improvement, the introduction of hydrophilic sulfonic acid groups, the water solubility has been significantly improved. And in the form of sodium salts, the same water solubility kept.

3. Water solubility of luminescent reagents

Luminol reagents are mainly luminol, isoluminol or direct luminescence acridine ester catalyzed by enzyme substances, but luminol or isoluminol have low solubility in neutral water, usually dissolved in alkaline In sodium hydroxide solution, but if luminol or isoluminol is made into sodium salt form, it can also be directly dissolved in neutral aqueous solution.

However, for acridine esters, it is not a question of whether they are soluble in water. The structure of acridine esters is complicated, and there is the possibility of hydrolysis in water. For example, the NHS contained in acridine esters has high activity and will be hydrolyzed in water. , but can be manipulated in aqueous solution after labeling the protein.

After numerous improvements and technological breakthroughs, the buffers, chromogenic reagents and luminescent reagents produced and developed by Desheng have good water solubility, stable performance and a purity of up to 99%, which can meet the requirements of various biochemical tests. The daily production capacity of the products has reached the ton level to ensure the supply of goods for customers. If you have requirements for diagnostic reagents, please click on the website to consult the details!