What's the Differences of HEPES and PIPES Buffers?

Both HEPES and PIPES are buffers, but they are fundamentally different. Of course, their roles in the application field are also different. As a zwitterionic buffer, HEPES has strong water solubility and is widely used in cell culture and diagnostic kits. In addition, because HEPES can maintain pH stability in biochemical reactions, it is often paired with fruit acids and used in cosmetics, such as water emulsions, gels, essences, etc., which can exfoliate and promote permeability. Helps protect skin whitening and translucent. However, PIPES is a buffer that is insoluble in water but soluble in aqueous sodium hydroxide solution. It cannot form stable complexes with various metal ions, so it is suitable for buffers in solution systems containing metal ions. Mainly it is widely used to purify proteins using chromatography as well as gel filtration to purify recombinant GTP, which binds proteins ARF1 and ARF2. On the other hand, it is not suitable for redox systems to crystallize transketolase from E. coli as a buffer. The performance difference between HEPES and PIPES In terms of water solubility, HEPES has strong water solubility, and its pH buffer range is neutral to alkaline, while PIPES cannot dissolve in aqueous solution, and the buffer range is also very different. In terms of the structural difference between the two, HEPES contains one so3h and -H, while PIPES has two so3h. Although PIPES and HEPES are both buffers, there are differences in various aspects. Therefore, when choosing, you should also consider the suitability of the experiment according to your own needs. Desheng Biochemical has specialized in R&D and production of buffers for many years. It is an established manufacturer with a professional R&D department and testing department to ensure product quality in all aspects. The biological buffer products currently produced include TRIS, TAPS, CAPS, BICINE, EPPS, HEPES, PIPES, MOPS, etc., which can meet the needs of different customers. They are available from stock, and the price is favorable for large quantities, ensuring your satisfaction. At present, the series of products produced by Desheng have been sold to more than 100 countries around the world, with perfect pre-sales and after-sales service, one-stop procurement service, and professionally answering procurement problems for you. If you have any needs, please click the website or inquire!