Potassium oxalate anticoagulant few people known

In the vacuum blood collection tube, there are many reagents that can be added, such as serum separation gel, anticoagulant, coagulant, etc. Among them, the anticoagulant includes heparin series and EDTA series products. However, in addition to these series of anticoagulants, there is also an anticoagulant "potassium oxalate", which is relatively rare but widely used. Few people in life know the anticoagulation principle of it as an anticoagulant, so here is the focus.

In fact, the obvious advantage of potassium oxalate as an anticoagulant is in solubility. After blood collection, potassium oxalate in the test tube dissolves and separates oxalate and reacts with calcium ions in the blood sample to form calcium oxalate precipitation. coagulation effect.

The use of potassium oxalate for anticoagulation is generally used at a concentration of 0.1mol/L, which is used in a ratio of 1:9 to the blood. However, due to the high concentration of potassium ions and sodium ions, it is easy to cause dehydration and shrinkage of blood cells, and ammonium ions can cause blood cells. Therefore, the proportion of potassium oxalate anticoagulant and blood in the test tube must be properly adjusted when measuring hematocrit without disturbing the shape and volume of red blood cells.

However, it should be noted that although potassium oxalate is often used in blood tests, it cannot be used to measure all components in blood. Platelet count, as it aggregates platelets and affects white blood cell morphology.

Potassium oxalate produced by Desheng is in powder form. It is prepared into a solution with a volume of 0.2ml and a concentration of 10% and is added to the test tube. Then put the test tube with the solution uniform in the oven below 80 ℃ to dry.

At this time, the drying temperature in the oven should not be too high. If it is too high, potassium oxalate will be decomposed into potassium carbonate, which will lose the anticoagulation effect. Therefore, the temperature must be controlled. In routine blood testing and analysis, potassium oxalate is commonly used as an anticoagulant in test tubes. Usually, fresh blood samples just collected can be stored for one year below -20°C.

Desheng Biochemical has been making blood collection tube additives for 17 years. As an old-fashioned manufacturer, it puts the interests of customers first and strictly controls the quality. At present, there are a large number of serum separation gels, anticoagulants, and coagulants. It is widely used in clinical medicine and in vitro diagnostic reagents, and has been praised by many partners. If you have the needs of blood collection tube additives, you can click the official website to consult!