In the electrophoresis experiment, a buffer is used to maintain the necessary conditions for a stable electrophoresis system to maintain a constant pH value, because during the electrophoresis experiment, the positive electrode and the negative electrode will undergo an electrolysis reaction, the positive electrode will undergo oxidation reaction, and the negative electrode will undergo a reduction reaction. Long-term electrophoresis will make the positive electrode become acidic, and the negative electrode will become alkaline. The buffer can keep the pH of the solution at the two poles basically unchanged. It will also make the solution have a certain degree of conductivity, which is conducive to the migration of DNA molecules.
Speaking of this, many people have thought of the biological buffer MOPS 3-morpholinopropanesulfonic acid, which belongs to Good's buffer, has good solubility in water, and the buffer range is between 6.5-7.9, which can be used as a two-dimensional gel electrophoresis. Electrolyte system component of IEF (IEF); it can also be used in Northern hybridization as a buffer for RNA separation and membrane transfer.
The commonly used 10×MOPS Buffer preparation methods in electrophoresis buffer are as follows:
(1) Weigh 41.8 g MOPS and dissolve it in about 700mL DEPC treated water;
(2) Use 2 N NaOH to adjust the pH to 7.0;
(3) Add 20 mL of DEPC-treated 1M NaOAC and 20 mL of 0.5M EDTA (pH 8.0) to the solution;
(4) Dilute the volume to 1 L, filter to remove impurities with a 0.45 μm filter membrane, and store at room temperature away from light.
In addition to MOPS buffer, there are many biological buffers used in electrophoresis, and there are certain differences between them, which are suitable for various electrophoresis. In the current market, there are many vendors of biological buffers, and product specifications and quality are uneven.
Desheng is a manufacturer specializing in the production of biological buffers. The biological buffer products currently produced include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PEP, etc. It has been established for more than ten years. With very rich R&D and production experience and product knowledge, we can provide customers with a large amount of technical support and after-sales guarantee. Please feel free to contact us if necessary!