What is the difference between Tris, Tris-base and TRIS base?
This problem has always been plagued by many scientific researchers. The scientific name of Tris is Tris, which is the same substance as Tris base and Tris base. The Chinese name is tris(hydroxymethyl)aminomethane; he has other aliases: tromethamine, tromethamine, which is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.
What is the connection between Tris and Tris-HCL?
1. Simply put, Tris is an alkaline solution, and Tris-HCL is an acidic solution. Remember that buffers are used to resist changes in pH. Even if there is no buffer, even a small amount of strong acid or base may significantly change the pH of the environment. Therefore, the reason for choosing between Tris-HCL or Tris also depends on the goal achieved by the buffer to be used.
2.Tris-HCl is configured with tris-hydroxymethylaminomethane and hydrochloric acid, and then the pH can be adjusted. Each PH segment is used. For protein experiments, PH6.8 and PH8.8 are more commonly used. Laboratory use is also used in protein, DNA, RNA experiments. Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses.
3. Sometimes TRIS and TRIS-HCL are also used in combination. If you extract the tissue protein of the animal, you may need to wash it with cold PBS (N contains TRIS) buffer at first, and then grind and extract the protein. There is Tris-HCl as a buffer, and PBS is not suitable at this time.
How to prepare Tris-HCl buffer
Use the following simple steps to prepare any Tris-HCl buffer at any molar concentration in this pH range
1) Calculate the number of moles of Tris Base: mole/liter * L = number of moles required
2) Calculate the mass of Tris Base: multiply the number of moles to determine the mass of Tris base to be weighed. The molecular weight of Tris (121.14 g/mol), the number of moles required * g / mol = g
3) Dissolve the Tris base in water: Dissolve the required quality of Tris in a certain volume of deionized water, about 1/3 of the volume of the buffer to be prepared.
4) Adjust the pH value: Use a pH meter to titrate the Tris solution with 1M hydrochloric acid (HCl) until the correct pH value is reached.
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