天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Journal of Agricultural and Food Chemistry >>article
Journal of Agricultural and Food Chemistry

Journal of Agricultural and Food Chemistry

IF: 5.7
Download PDF

4-Methylumbelliferone Inhibits Angiogenesis in Vitro and in Vivo

Published:12 April 2013 DOI: 10.1021/jf303062h PMID: 23581646
Javier A. García-Vilas, Ana R. Quesada, Miguel ángel Medina*

Abstract

4-Methylumbelliferone (4-MU) is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects. The objective of the present study was to determine the potential of 4-MU as an antiangiogenic compound. To fulfill this aim, cultured endothelial cells were used to perform an array of in vitro assays, as well as two different in vivo angiogenesis assays. This study demonstrates that, in fact, 4-MU behaves as a new inhibitor of both in vitro and in vivo angiogenesis. In vitro, 4-MU affects several key steps of angiogenesis, including endothelial cell proliferation, adhesion, tube formation, and extracellular matrix remodeling. Half-maximal inhibitory concentrations (IC50) values in the proliferation assay were 0.65 ± 0.04 and 0.37 ± 0.03 mM for HMEC and RF-24 endothelial cells, respectively. 4-MU (2 mM) treatment for 24 h induced apoptosis in 13% of HMEC and 5% of RF-24 cells. The number of adherent endothelial cells decreased by >20% after 24 h of treatment with 1 mM 4-MU. Minimal inhibitory concentrations in the tube formation assay were 2 and 0.5 mM 4-MU for HMEC and RF-24, respectively. Matrix metalloproteinase-2 expression was differentially altered upon 4-MU treatment in both tested endothelial cell lines. Taken together, the results suggest that 4-MU may have potential as a new candidate multitargeted bioactive compound for antiangiogenic therapy.

Substances (2)

Related products
Procduct Name CAS Molecular Formula Supplier Price
4-Methylumbelliferone 90-33-5 C10H8O3 664 suppliers $9.00-$801.00
Hyaluronic acid 9004-61-9 C14H22NNaO11 612 suppliers $60.00-$1174.00

Similar articles

IF:3.8

Salidroside inhibits the growth of human breast cancer in vitro and in vivo.

Oncology reports Gang Zhao, Aiping Shi,etc Published: 1 May 2015
IF:4.6

In Vitro and In Vivo Neuroprotective Effects of Sarcosine.

ACS Applied Bio Materials Arzugül Tanas, ?zlem ?zdemir Tozlu,etc Published: 15 October 2022
IF:8.3

4-methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy in inflammation, autoimmunity, and cancer.

ACS Applied Materials & Interfaces Nadine Nagy, Hedwich F Kuipers,etc Published: 23 March 2015