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Journal of Food Science

Journal of Food Science

IF: 3.2
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Macroporous resins combined with UPLC‐QTRAP‐MS/MS for the purification and identification of 3‐deoxyanthocyanidins from Sorghum bicolor L. Moench and their bioactivities

Published:24 November 2024 DOI: 10.1111/1750-3841.17526 PMID: 39581623
Yanbei Wu, Xueling Yu, Wei Ding, Mengmeng Xin, Xu Zhang, Lulu Wang, Huan Wang, Jing Wang

Abstract

3-Deoxyanthocyanidins (3-DAS) are the unique flavonoid compounds in sorghum. The majority of research to date have concentrated on the biological activity and extraction of 3-DAS from sorghum and lacked systematic purification and identification investigations. Herein, a facile method for the purification of sorghum 3-DAS from the acidic methanol solution crude extract using macroporous resins (MARs) was proposed and investigated in this work. Based on the experimental data, commercial MAR AB-8 was selected to achieve the purification of sorghum 3-DAS. The adsorption-desorption experiment confirmed the optimal procedure. With this procedure, the total purity of 3-DAS was 19.32%, and 3-DAS content in the purified product was 8.4 times higher than that in the crude extract. UPLC-QTRAP-MS/MS identification indicated that the purified 3-DAS product was mainly composed of apigeninidin, luteolinidin, and their methoxy derivatives. Besides, the purified product exhibited better bioactivities than the crude extract, including the scavenging activities for DPPH, ABTS+, and hydroxyl radicals, the total antioxidant capacity, and the inhibitory effects for α-amylase, α-glucosidase, and lipase. This work provides a potential way for the large-scale purification of bioactive sorghum 3-DAS from crude extract, which is significant to deepen the applications of sorghum 3-DAS in the food nutrition and pharmaceutical industries.

Substances (2)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Lipase 9001-62-1 C11H9N3NaO2+ 505 suppliers $30.20-$2150.00
α-Glucosidase 9001-42-7 NULL 213 suppliers $66.10-$562.00