英文名稱(chēng)： Sulfo-NHS-Acetate
3-Sulfosuccinimid-1-yl acetate
1-Acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid
中文名稱(chēng)：1-乙酰氧基-2,5-二氧代吡咯烷-3-磺酸
CAS NO： 152305-87-8
MDL Number：MFCD01861959
分子式： C6H6NNaO7S
分子量： 259.2
純 度： ≥95%
結(jié)構(gòu)式:
性 狀:白色或微黃色粉狀制劑
用 途：僅供科研實(shí)驗(yàn)使用，不用于診治
注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干
溶解性：溶于大部分有機(jī)溶劑，如：DCM、DMF、DMSO、THF等等。在水中有很好的溶解性。
簡(jiǎn) 介：蛋白質(zhì)交聯(lián)劑是一類(lèi)小分子化合物，具有2個(gè)或者更多的針對(duì)特殊基團(tuán)（-NH2、-COOH、-HS等）的反應(yīng)性末端，可以和2個(gè)或者更多的分子分別偶聯(lián)，從而使這些分子結(jié)合在一起，終形成共軛蛋白。
1. Dissolve protein at a concentration of 1 – 10 mg/mL in 100 mM sodium phosphate buffer, pH 7.0- 8.0. Avoid amine containing buffers, such as Tris, glycine, or imidazole, etc.
2. Create a 50 mg/mL Sulfo-NHS-Acetate stock solution in buffer (same buffer as in step one).
3. To the protein solution, add 2 μL of the Sulfo-NHS-Acetate stock for every mg of protein dissolved. (ie. 10mg of protein = 5 μL Sulfo-NHS-Acetate stock). Note: Alternatively, you can add an equivalent amount of Sulfo-NHS-Acetate to the protein solution (mg of protein = mg of Sulfo-NHS-Acetate).
4. Allow this reaction to proceed for 1-2 hours at room temperature. (2-3 hours if protein stability issues require 4°C incubation).
5. If desired, residual Sulfo-NHS-Acetate can be deactivated by adding 0.5M Tris-HCl, pH 7.4 – 8.0 or 0.5M glycine to this reaction mixture. This is redundant if removing Sulfo-NHS-Acetate by desalting (see step 6).
6. Desalt sample to remove residual Sulfo-NHS-Acetate and reaction bi-products (i.e. gel filtration, dialysis, etc.). Note: As a general rule for amine blocking with Sulfo-NHS-Acetate, 10-50 molar excess of SulfoNHS-Acetate to amines to be blocked will provide sufficient coverage.
產(chǎn)品列表：
Sulfo-NHS CAS #: 106627-54-7
BS3 CAS #: 82436-77-9
BS2G
BSG CAS #: 79642-50-5
DSP CAS #: 57757-57-0
DSS CAS #: 68528-80-3
DTSSP CAS #: 81069-02-5
EGS CAS #: 70539-42-3
Sulfo-EGS
DSSeb CAS # 23024-29-5
BMPS CAS #: 55750-62-4
EMCS CAS #: 55750-63-5
GMBS CAS #: 80307-12-6
LC-SPDP
MBS CAS #: 58626-38-3
PDPH
SBA CAS #: 42014-51-7
SIA CAS #: 39028-27-8
SMCC CAS #: 64987-85-5
SMPB CAS #: 79886-55-8
SMPH
SPDP CAS #: 68181-17-9
Sulfo-LC-SPDP
Sulfo-MBS
Sulfo-SANPAH CAS #: 102568-43-4
Sulfo-SMCC CAS #: 92921-24-9
BS2G-d4
BS3-d4
DSG-d4
DSS-d4
DSP-d8
Biotin Hydrazide CAS #: 66640-86-6
NHS-Biotin CAS #: 35013-72-0
NHS-SS-Biotin CAS #: 142439-92-7
Sulfo-NHS-Biotin CAS #: 119616-92-5
Sulfo-NHS-LC-Biotin CAS #: 128062-22-0
Sulfo-NHS-SS-Biotin CAS #: 202057-28-1
HPG CAS #: 24645-80-5
SATA CAS #: 76931-93-6
Sulfo-NHS Acetate CAS #: 152305-87-8