Stemgent⑧Purified Mouse anti-Human c-Myc Antibody詳細(xì)信息: Product Overview Stemgent Purified Mouse anti-Human c-Myc Antibody was screened on cells transfected with a human C-Myc construct by western blot analysis and selected as the best c-Myc antibody, among those tested, for stem cell research applications. The c-Myc protein is a 62 kD transtription factor that is believed to regulate expression of 15% of all genes1-2. c-Myc is activated upon by various mitogenic signals such as Wnt, SHH and EGF (via the MAPK/ERK pathway). By modifjing the expression of its target genes, c-Myc activation results in numerous biological effects such as cell proliferation (upregulation of cyclins, downregulation of p21), regulation of cell growth (upregulation of ribosomal RNA and proteins), apoptosis (downregulation of Bcl-2), and differentiation3. C-Myc is a very strong proto-oncogene and is very often found to be upregulated in many types of cancers. Therefore C-Myc inhibitors have a great potential for cancer therapy4. C-Myc is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state5-6. Stemgent Purified Mouse anti-Human c-Myc AntibodyProduct Specifications Size 1 Quantity 100 μl (0.5 mg/ml) Immunogen C-terminal region of human C-Myc (amino acids 408- 439) Reactivity Human Clone 9E10 Isotype Mouse lgG1, | Formulation Phosphate -buffered solution pH 7.2 containing≤0.09% sodium azide. Preparation The antibody was purified by affinity chromatography. Storage Store undiluted at 4°C. Do not freeze. Stability Stable for 6 months from date of receipt when stored as directed. Quality Control Tested by western blot analysis Recommended Usage Dilute 1:200 to 1:500 for western blotting applications. For a general protocol for performing western blot analysis directly on stem cells, see Technical Documents.
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