The pET-47b(+) plasmid features the ability to express fusion proteins with an N-terminal His•Tag® coding sequence that is cleavable with the human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification.