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pRetroX-IRES-ZsGreen1 載體,pRetroX-IRES-ZsGreen1
  • pRetroX-IRES-ZsGreen1 載體,pRetroX-IRES-ZsGreen1

pRetroX-IRES-ZsGreen1 載體

價格 1280
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2025-02-08
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產(chǎn)品詳情

中文名稱:pRetroX-IRES-ZsGreen1 載體英文名稱:pRetroX-IRES-ZsGreen1
保存條件: 常溫運輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2025-02-08 pRetroX-IRES-ZsGreen1 載體 pRetroX-IRES-ZsGreen1 2ug/1280RMB 1280 常溫運輸 99% 質(zhì)粒/載體

pRetroX-IRES-ZsGreen1 載體

質(zhì)粒類型:
逆轉(zhuǎn)錄病毒載體
高拷貝/低拷貝:
高拷貝
克隆方法:
多克隆位點,限制性內(nèi)切酶
載體大小:
5953bp (查看載體序列)
3' 測序引物及序列:
IRES-R: CCTCACATTGCCAAAAGACG
載體抗性:
Ampicillin (氨芐青霉素)
備注:
載體表達ZsGreen綠色熒光蛋白作為篩選標志,用來構(gòu)建穩(wěn)表達細胞系。
產(chǎn)品編號
產(chǎn)品名稱
規(guī)格
價格
ZY1542
pRetroX-IRES-ZsGreen1
2ug
 
pRetroX-IRES-ZsGreen1 質(zhì)粒圖譜
pRetroX-IRES-ZsGreen1 載體圖譜

pRetroX-IRES-ZsGreen1 is a bicistronic, fl uorescent, retroviral vector that allows both a gene of interest and the ZsGreen1 gene to be translated from a single bicistronic mRNA. pRetroXIRES- ZsGreen1 is designed for effi cient delivery and selection (by fl ow cytometry or other methods) of stably transduced mammalian cells expressing the ZsGreen1 fl uorescent protein and the protein of interest. This vector can be used to obtain stable cell lines without timeconsuming drug and clonal selection. ZsGreen1 is derived from the reef coral Zoanthus sp. green fl uorescent protein (ZsGreen) and is easily detected with standard FITC fi lter sets (1).

Bicistronic expression from this vector is facilitated by the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). This IRES facilitates cap-independent translation from an internal start site at the IRES/ZsGreen1 junction (2). ZsGreen1 is a human codon optimized ZsGreen variant that encodes the brightest commercially available green fl uorescent protein (1). This retroviral vector is derived from the pMIN series of vectors (3, 4). These optimized vectors have the ability to produce high viral titers, express genes at high levels, and, due to the absence of retroviral coding sequences, exhibit improved safety profi les. The multiple cloning site (MCS) in pRetroX-IRES-ZsGreen1 is between the 5’ MMLV LTR and the IRES sequence. Genes cloned into the MCS are expressed as a bicistronic message transcribed from the 5’ LTR.

pRetroX-IRES-ZsGreen1 contains all of the necessary viral RNA processing elements; these include the 5’ and 3’ LTRs, the packaging signal (ψ), and the tRNA primer-binding site. For safety reasons, however, the vector lacks the structural genes (gag, pol, and env) necessary for retroviral particle formation and replication. pRetroX-IRES-ZsGreen1 contains a ColE1 origin of replication, and an E. coli Ampr gene for propagation and selection in bacteria.

載體應(yīng)用

pRetroX-IRES-ZsGreen1 is designed to effi ciently deliver and co-express your gene of interest and ZsGreen1 in any mitotically-active mammalian cell. When cloning into pRetroX-IRES-ZsGreen1, your gene of interest should contain an initiation codon (ATG) and a stop codon. The vector can be infected or transfected into mammalian cells. If required, stable transformants can be selected by fl ow cytometry or limiting dilution.

In order to infect mammalian cells with pRetroX-IRES-ZsGreen1, the vector must be transfected into a packaging cell line, such as the RetroPack™ PT67 Cell line (631510), AmphoPack™-293 (631505), EcoPack™2- 293 (631507), Pantropic Expression System (631512), or Retro-X™ Universal Packaging System (631530). These cell lines package RNA from the vector into infectious, replication-incompetent, retroviral particles. Such retroviral particles can infect target cells and transmit the gene of interest, but cannot replicate within these cells due to the absence of viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chance of producing replication-competent virus due to recombination events during cell proliferation.

特別提示:本公司的所有產(chǎn)品僅可用于科研實驗,嚴禁用于臨床醫(yī)療及其他非科研用途!

關(guān)鍵字: pRetroX-IRES-ZsGreen1 載體/pRetroX-IRES-ZsGreen1質(zhì)粒

公司簡介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測試劑等等
成立日期 2016-08-08 (9年) 注冊資本 100萬元整
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 生物化工,有機原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 6年
  • 公司成立:9年
  • 注冊資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營產(chǎn)品:主營產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標準品、分子生物學(xué)試劑、細胞生物學(xué)等科研用品
  • 公司地址:松江區(qū)新橋鎮(zhèn)賣新公里2077號8309室
詢盤

pRetroX-IRES-ZsGreen1 載體相關(guān)廠家報價

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詢價
VIP6年
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2025-02-09
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