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pRevTet-On 載體,pRevTet-On
  • pRevTet-On 載體,pRevTet-On

pRevTet-On 載體

價(jià)格 990
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2024-12-25
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產(chǎn)品詳情

中文名稱(chēng):pRevTet-On 載體英文名稱(chēng):pRevTet-On
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類(lèi)別: 質(zhì)粒/載體
2024-12-25 pRevTet-On 載體 pRevTet-On 2ug/990RMB 990 常溫運(yùn)輸 99% 質(zhì)粒/載體

pRevTet-On 載體

質(zhì)粒類(lèi)型:
四環(huán)素調(diào)控系統(tǒng)
啟動(dòng)子:
CMV
克隆方法:
多克隆位點(diǎn),限制性?xún)?nèi)切酶
載體大小:
7.6kb
載體抗性:
Ampicillin (氨芐青霉素)
篩選標(biāo)記:
Neomycin(新霉素)
產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
規(guī)格
價(jià)格
ZY1611
pRevTet-On
2ug
 
pRevTet-On 質(zhì)粒圖譜

pRevTet-On is a retroviral vector expressing the reverse tetracycline-controlled transactivator (rtTA) from the CMV promoter. This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described (1). rtTA is a fusion of amino acids 1–207 of the reverse tet repressor (rTetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of Herpes Simplex Virus. rTetR was derived from TetR and differs by four point mutations, which are responsible for its opposite response to doxycycline. The 5' viral LTR controls expression of the transcript that contains Ψ+ (the extended viral packaging signal) and the neomycin resistance gene (Neor) for antibiotic selection in mammalian cells. rtTA is derived from vectors described previously (2–4). pRevTet-On also includes the E. coli Ampr gene for antibiotic selection in bacteria.

載體應(yīng)用

pRevTet-On can be used to establish stable Tet-On cell lines via retrovirus-mediated gene transfer (5). Retroviral gene transfer allows the highly efficient transduction of virtually all dividing cell types. The RevTet Systems are also suitable for establishing transgenic animals. In combination with the pRev-TRE retroviral expression vector, a gene of interest can be inducibly expressed at high levels in response to varying concentrations of the tetracycline derivative doxycycline (Dox). rtTA binds to the Tet-response element (TRE), thus activating transcription in the presence of Dox. The response of rtTA to Dox is thus opposite to the response of tTA. As Dox is removed from the culture medium, transcription from the inducible promoter is turned off in a highly dose-dependent manner. pRevTet-On lacks the viral genes gag, pol, and env, which are supplied by the packaging cell line. It can be transfected into a high titer packaging cell line and thereby mediate production of infectious, replication-incompetent retroviral particles (1, 6–7).The transcript produced by the pRevTet-On construct is recognized by the viral structural proteins expressed in a packaging cell line and packaged into infectious retroviral particles. Because the RNA transcript packaged in these particles does not contain the viral genes, it cannot replicate in the target cells that it infects.

The level of induction in cell populations infected with this vector depends on the efficiency of infection, the site of integration, and the titer of the virus. Viral supernatants with titers >105 cfu/ml should be produced to achieve high-level induction.

特別提示:本公司的所有產(chǎn)品僅可用于科研實(shí)驗(yàn),嚴(yán)禁用于臨床醫(yī)療及其他非科研用途!

關(guān)鍵字: pRevTet-On 載體/pRevTet-On質(zhì)粒

公司簡(jiǎn)介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開(kāi)發(fā)研究提供所需要的科研類(lèi)試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測(cè)試劑等等
成立日期 2016-08-08 (9年) 注冊(cè)資本 100萬(wàn)元整
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬(wàn)以?xún)?nèi)
主營(yíng)行業(yè) 生物化工,有機(jī)原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營(yíng)模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 6年
  • 公司成立:9年
  • 注冊(cè)資本:100萬(wàn)元整
  • 企業(yè)類(lèi)型:貿(mào)易
  • 主營(yíng)產(chǎn)品:主營(yíng)產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
  • 公司地址:松江區(qū)新橋鎮(zhèn)賣(mài)新公里2077號(hào)8309室
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