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pBI Tet 載體,pBI Tet
  • pBI Tet 載體,pBI Tet

pBI Tet 載體

價(jià)格 990
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2024-12-23
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產(chǎn)品詳情

中文名稱:pBI Tet 載體英文名稱:pBI Tet
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2024-12-23 pBI Tet 載體 pBI Tet 2ug/990RMB 990 常溫運(yùn)輸 99% 質(zhì)粒/載體

pBI Tet 載體

質(zhì)粒類型:
四環(huán)素調(diào)控系統(tǒng)
載體大小:
4.4kb
載體抗性:
Ampicillin (氨芐青霉素)
產(chǎn)品編號
產(chǎn)品名稱
規(guī)格
價(jià)格
ZY1601
pBI Tet
2ug
 
pBI Tet質(zhì)粒圖譜

The pBI Tet Vector is a response plasmid that can be used to express two genes of interest from one bidirectional tet-responsive promoter (Pbi-1; 1) in Clontech’s Tet-On® and Tet-Off® Gene Expression Systems and Cell Lines (2). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (3; Tet-Off) and Gossen et al. (4; Tet-On). The pBI Tet Vector contains the bidirectional promoter Pbi-1 which is responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. Pbi-1 contains the Tet-responsive element (TRE), which consists of seven copies of the 42-bp tet operator sequence (tetO). The TRE element is between two minimal CMV promoters (PminCMV), which lack the enhancer that is part of the complete CMV promoter. Consequently, Pbi-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. PminCMV-1 and PminCMV-2 control the expression of two separate genes of interest. Note that the cloned inserts must have an initiation codon. In some cases, addition of a Kozak consensus ribosome binding site (5) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence.

載體應(yīng)用

pBI allows the simultaneous regulation of two genes of interest by one central TRE. After a stable Tet-On or Tet-Off cell line has been established by transfecting with a tTA or rtTA regulator plasmid, pBI is cotransfected with pTK-Hyg (Cat No. 631750) to permit selection of a double-stable cell line which expresses both genes of interest. Alternatively, pPUR (Cat No. 631601) or another selection plasmid can be used. If this plasmid contains an enhancer element, as does pPUR, cointegration of pBI and the selection plasmid may lead to higher background expression. Double-stable, tet-responsive cell lines with pBI response constructs can be developed using the protocols described for pTRE response plasmids in the Tet Systems User Manual (PT3001-1).

特別提示:本公司的所有產(chǎn)品僅可用于科研實(shí)驗(yàn),嚴(yán)禁用于臨床醫(yī)療及其他非科研用途!

關(guān)鍵字: pBI Tet 載體/pBI Tet質(zhì)粒

公司簡介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測試劑等等
成立日期 2016-08-08 (9年) 注冊資本 100萬元整
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 生物化工,有機(jī)原料,化學(xué)試劑,醫(yī)藥原料,技術(shù)服務(wù) 經(jīng)營模式 貿(mào)易,試劑,定制,服務(wù)
  • 上海澤葉生物科技有限公司
VIP 6年
  • 公司成立:9年
  • 注冊資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營產(chǎn)品:主營產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
  • 公司地址:松江區(qū)新橋鎮(zhèn)賣新公里2077號8309室
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