Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase (Crude Enzyme)
Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase，中文名為谷氨酰半胱氨酸合成/谷胱甘肽合成雙功能酶。本產(chǎn)品為大腸桿菌表達(dá)的谷氨酰半胱氨酸合成/谷胱甘肽合成雙功能酶，并經(jīng)簡(jiǎn)單純化獲得的可以確保達(dá)到一定酶活力的粗酶液。
由于本產(chǎn)品需新鮮制備，確認(rèn)訂購(gòu)后約6個(gè)工作日才可以發(fā)貨。個(gè)別情況首次制備出現(xiàn)問(wèn)題，發(fā)貨時(shí)間會(huì)順延。
本產(chǎn)品可以提供十公斤、百公斤乃至噸級(jí)的粗酶液，如有需求請(qǐng)聯(lián)系康朗。
GSH, and by extension GCL, is critical to cell survival. Nearly every eukaryotic cell, from plants to yeast to humans, expresses a form of the GCL protein for the purpose of synthesizing GSH. To further highlight the critical nature of this enzyme, genetic knockdown of GCL results in embryonic lethality. Furthermore, dysregulation of GCL enzymatic function and activity is known to be involved in the vast majority of human diseases, such as diabetes, Parkinson's disease, Alzheimers disease, COPD, HIV/AIDS, and cancer. This typically involves impaired function leading to decreased GSH biosynthesis, reduced cellular antioxidant capacity, and the induction of oxidative stress. However, in cancer, GCL expression and activity is enhanced, which serves to both support the high level of cell proliferation and confer resistance to many chemotherapeutic agents.Glutathione synthase belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). This product with the indicated enzyme activity was briefly purified from engineered E.coli.
產(chǎn)品詳細(xì)信息見(jiàn)下表:
Name
Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase (Crude Enzyme)；谷氨酰半胱氨酸合成/谷胱甘肽合成雙功能酶(粗酶液)
Appearance
Clear to translucent yellow solution
Enzyme activity
Undetermined
Name
Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase；谷氨酰半胱氨酸合成/谷胱甘肽合成雙功能酶
CAS Number
9023-64-7/9023-62-5
EC Number
6.3.2.2/ 6.3.2.3
Enzymatic Reaction
L-glutamate + L-cysteine + ATP ? γ-L-glutamyl-L-cysteine + ADP + phosphate
ATP + γ-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione
Systematic name
L-glutamate:L-cysteine γ-ligase (ADP-forming)
γ-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
Application
agriculture; medicine; synthesis; biotechnology; pharmacology
包裝清單：
產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
包裝
KL3829-100ml
Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase (Crude Enzyme)
100ml
KL3829-1L
Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase (Crude Enzyme)
1L
保存條件：
-20℃或更低溫度保存，至少1個(gè)月有效。
注意事項(xiàng)：
由于本產(chǎn)品需新鮮制備，在您確認(rèn)訂購(gòu)后約6個(gè)工作日才能發(fā)貨。個(gè)別情況首次制備出現(xiàn)問(wèn)題，發(fā)貨時(shí)間會(huì)順延。
粗酶液的每次凍融均可能會(huì)引起部分失活，所以收到酶液后請(qǐng)根據(jù)需要進(jìn)行分裝，并置于-20℃或更低溫度保存。
隨著保存時(shí)間的延長(zhǎng)，酶活力會(huì)有一定程度的下降，收到產(chǎn)品后應(yīng)盡快使用。
本產(chǎn)品在生產(chǎn)和保存的過(guò)程中可能會(huì)有混濁或沉淀產(chǎn)生，融化后混勻即可，不影響正常使用。
本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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關(guān)鍵字： Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase (Crude Enzyme)說(shuō)明書(shū)；Bifunctional Chimeras of Glutamylcysteine Synthetase and Glutathione Synthetase (Crude Enzyme)廠家
上海康朗生物科技有限公司是一家集研發(fā)、生產(chǎn)、銷(xiāo)售、服務(wù)于一體的一家生物科技企業(yè)，專(zhuān)營(yíng)生化試劑、標(biāo)準(zhǔn)品、基因、蛋白、抗體、Elisa試劑盒、細(xì)胞生物學(xué)，分子生物學(xué)等高生物產(chǎn)品?？偛课挥谏虾＃⒃诒本?、廣東，江西，吉林等全國(guó)30多個(gè)省市設(shè)有分公司和代理機(jī)構(gòu)。涉及的產(chǎn)品被中國(guó)科學(xué)院、清華、北大、復(fù)旦，上海交大，復(fù)旦醫(yī)學(xué)院，上海中醫(yī)藥大學(xué)，華東師范大學(xué)，第二軍醫(yī)大學(xué)，曙光醫(yī)院，浦東新區(qū)人民醫(yī)院等知名科研院所廣泛使用，可靠而穩(wěn)定的質(zhì)量和完善的售后服務(wù)確。