Name

Recombinant Murine SHH (Recombinant Murine Sonic Hedgehog N-Terminus; rMuSHH) ; Recombinant Mouse SHH ; 重組小鼠音猬因子

Synonyms

Shh; HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog; sonic hedgehog homolog; sonic hedgehog protein; TPT; TPTPS

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5-1.0μg/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuSHH as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and indian hedgehog (IHH). As the hh homologues, SHH has been found to have the most critical roles in development. It acts as a morphogen involved in patterning many systems, including the limb and midline structures in the brain, spinal cord, the thalamus by the zona limitans intrathalamica and the teeth. There SHH undergoes auto-processing to generate a 20kDa (residues 24-197) N-terminal signaling domain (SHH-N) and a 25kDa (residues 198-462) C-terminal domain with no known signaling role.

Amino Acid Sequence

IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

產(chǎn)品編號

產(chǎn)品名稱

包裝

KL7325-5μg

Recombinant Murine SHH

5μg

KL7325-25μg

Recombinant Murine SHH

25μg

KL7325-100μg

Recombinant Murine SHH

100μg

KL7325-1mg

Recombinant Murine SHH

1mg

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說明書

1份