Name

Recombinant Human C5a (Recombinant Human Complement Component C5a; rHuC5a)；重組人C5a蛋白

Synonyms

C5D; C5a; C5b; ECLZB; CPAMD4

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1EU/μg of rHuC5a as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Complement C5, also named C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, is involved in the complement system and it is encoded by the C5 gene in human. Complement C5 is cleaved into C5a and C5b. C5a plays an important role in chemotaxis and C5b forms the first part of the complement membrane attack complex. Complement C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is composed of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.

Amino Acid Sequence

TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR

產(chǎn)品編號(hào)

產(chǎn)品名稱(chēng)

包裝

KL7313-5μg

Recombinant Human C5a

5μg

KL7313-20μg

Recombinant Human C5a

20μg

KL7313-100μg

Recombinant Human C5a

100μg

KL7313-1mg

Recombinant Human C5a

1mg

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說(shuō)明書(shū)

1份