Recombinant Human LR3 IGF-1, GMP
Name
Recombinant Human LR3 IGF-1, GMP (Recombinant Human LR3 Insulin-like Growth Factor-1, GMP; rHuLR3 IGF-1, GMP)；重組人LR3胰島素樣生長因子1
Synonyms
IGF1; IGF-1; Somatomedin A; Somatomedin C; IBP1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; somatomedin-C; Somatomedin-C
Purity
>98% by SDS-PAGE analyses. >90% by RP-HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10ng/ml, corresponding to a specific activity of >1.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.2.
Endotoxin
Less than 0.01EU/μg of rHuLR3 IGF-1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3 IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. Manufactured using all Animal-Free reagents.
Amino Acid Sequence
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
包裝清單:
XY-7489-1mg
Recombinant Human LR3 IGF-1, GMP
1mg
XY-7489-5mg
Recombinant Human LR3 IGF-1, GMP
5mg
XY-7489-50mg
Recombinant Human LR3 IGF-1, GMP
50mg
保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以首次配制成相應濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
使用說明：
1. 收到產(chǎn)品后請立即按照說明書推薦的條件保存。除非特別注明，Recombinant Human LR3 IGF-1, GMP
Name
Recombinant Human LR3 IGF-1, GMP (Recombinant Human LR3 Insulin-like Growth Factor-1, GMP; rHuLR3 IGF-1, GMP)；重組人LR3胰島素樣生長因子1
Synonyms
IGF1; IGF-1; Somatomedin A; Somatomedin C; IBP1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; somatomedin-C; Somatomedin-C
Purity
>98% by SDS-PAGE analyses. >90% by RP-HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10ng/ml, corresponding to a specific activity of >1.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.2.
Endotoxin
Less than 0.01EU/μg of rHuLR3 IGF-1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3 IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. Manufactured using all Animal-Free reagents.
Amino Acid Sequence
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
包裝清單:
KL-7489-1mg
Recombinant Human LR3 IGF-1, GMP
1mg
KL-7489-5mg
Recombinant Human LR3 IGF-1, GMP
5mg
KL-7489-50mg
Recombinant Human LR3 IGF-1, GMP
50mg
保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以首次配制成相應濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
使用說明：
1. 收到產(chǎn)品后請立即按照說明書推薦的條件保存。除非特別注明，康朗生物相關產(chǎn)品均為凍干粉，由于微量的蛋白在凍干過程中沉積在管內，形成很薄或不可見的蛋白層，所以在打開管蓋前，我們建議在離心機中約8,000-12,000g離心10-30秒，使附著在管蓋或管壁上的蛋白聚集于管底。
2. 請根據(jù)實驗目的并按照產(chǎn)品簡介中Reconstitution一欄中的信息配制儲存液。大多數(shù)細胞因子或重組蛋白的凍干粉是非常容易溶解的，一般用移液槍的槍頭輕吹幾下或者輕輕搖晃瓶子，即可使細胞因子或重組蛋白完全溶解。請勿用vortex劇烈振蕩，以免蛋白變性而失活。
3. 具體的工作濃度請自行參考相關文獻，或者根據(jù)實驗目的，以及特定細胞和動物，通過實驗進行摸索和優(yōu)化。
相關產(chǎn)品均為凍干粉，由于微量的蛋白在凍干過程中沉積在管內，形成很薄或不可見的蛋白層，所以在打開管蓋前，我們建議在離心機中約8,000-12,000g離心10-30秒，使附著在管蓋或管壁上的蛋白聚集于管底。
2. 請根據(jù)實驗目的并按照產(chǎn)品簡介中Reconstitution一欄中的信息配制儲存液。大多數(shù)細胞因子或重組蛋白的凍干粉是非常容易溶解的，一般用移液槍的槍頭輕吹幾下或者輕輕搖晃瓶子，即可使細胞因子或重組蛋白完全溶解。請勿用vortex劇烈振蕩，以免蛋白變性而失活。
3. 具體的工作濃度請自行參考相關文獻，或者根據(jù)實驗目的，以及特定細胞和動物，通過實驗進行摸索和優(yōu)化。
關鍵字： Recombinant Human LR3 IGF-1, GMP說明書；Recombinant Human LR3 IGF-1, GMP廠家
上?？道噬锟萍加邢薰臼且患壹邪l(fā)、生產(chǎn)、銷售、服務于一體的一家生物科技企業(yè)，專營生化試劑、標準品、基因、蛋白、抗體、Elisa試劑盒、細胞生物學，分子生物學等高生物產(chǎn)品?？偛课挥谏虾＃⒃诒本?、廣東，江西，吉林等全國30多個省市設有分公司和代理機構。涉及的產(chǎn)品被中國科學院、清華、北大、復旦，上海交大，復旦醫(yī)學院，上海中醫(yī)藥大學，華東師范大學，第二軍醫(yī)大學，曙光醫(yī)院，浦東新區(qū)人民醫(yī)院等知名科研院所廣泛使用，可靠而穩(wěn)定的質量和完善的售后服務確。