Name
Recombinant Murine sTNF RI/TNFRSF1A (Recombinant Murine soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A; rMusTNF RI/TNFRSF1A)；Recombinant Mouse sTNF RI/TNFRSF1A ; 重組小鼠可溶性腫瘤壞死因子受體I型/TNFRSF1A
Synonyms
CD120a; TNFRSF1A; CD120a; CD120a antigen; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1μg/ml, corresponding to a specific activity of >1000IU/mg in the presence of 0.1ng/ml of rMuTNF-α.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rMusTNF RI/TNFRSF1A as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
TNF RI, also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily and it is encoded by the TNFRSF1A gene in human. TNF RI is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF RI is one of the major receptors for the TNF-α. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Murine TNF RI shares 70%, 88%, 67%, 70% and 64%a.a. sequence identity with human, rat, canine, feline and porcine TNF RI, respectively.
Amino Acid Sequence
IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A