For the quantitative in vitro determination of Insulin concentrations in Mouse culture supernates, serum, plasma and tissue.
1.?Serum-Use a serum separator tube(SST) and allow samples to clot for 30minutes before?centrifugation for 15minutes at approximately 1000 xg.Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C.?
2.?Plasma-Collect plasma using EDTA or?Citric acid sodium?as an anticoagulant.Centrifuge?samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection. Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.?
3.?Cell culture fluid and other biological fluids-Remove particulates by centrifugation and?assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.樣本處理及要求
1. 血清-用采血管收集血液，室溫血液自然凝固30分鐘，1000 xg離心15分鐘左右，收集上清，盡早進(jìn)行實驗，或者分裝后-20°C 或 -80°C 保存
2. 血漿-收集好的血漿根據(jù)要求選擇EDTA或者檸檬酸鈉作為抗凝劑，2-8°C 1000 xg離心15分鐘左右，30分鐘內(nèi)收集好，-20°C 或 -80°C 保存，避免反復(fù)凍融
3. 細(xì)胞上清及相關(guān)液體-收集好的樣本離心后應(yīng)盡快實驗，或者分裝后-20°C 或 -80°C 保存，避免反復(fù)凍融