選擇性抗生素Blasticidin | invivogenblasticidin是一種高效的選擇性抗生素，作用于真核細胞和原核細胞。稻瘟菌素是一類肽核苷類抗生素鏈霉菌griseochromogenes，干擾在核糖體的肽鍵的形成，從而抑制蛋白質(zhì)的合成分離。

抗稻瘟是賦予了稻瘟抗性基因從Bacillus cereus（BSR），其編碼滅瘟素脫氨酶。

稻瘟菌素誘導細胞死亡和細胞快速進行稻瘟抗性基因可以在不到一周的時間了。

通常情況下，哺乳動物細胞對稻瘟μ1-10μg／ml濃度敏感，和細菌的25-100μ克/毫升

Product concentration: 10 mg/ml

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 95% (HPLC)
Endotoxin level: < 1 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in blasticidin-resistant cells
Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer. This product is available in four pack sizes:
- ant-bl-05: 5 x 1 ml (50 mg)
- ant-bl-1: 10 x 1 ml (100 mg)
- ant-bl-5: 50 x 1 ml (500 mg)
- ant-bl-5b: 1 x 50ml (500 mg)
Blasticidin hydrochloride is also supplied as a powder:
- ?ant-bl-10p: 1 g (powder)
Blasticidin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

推薦使用濃度
大腸桿菌
大腸桿菌對Blasticidin不敏感，但有Blasticidin抗性的轉(zhuǎn)化子可以在低鹽LB瓊脂糖培養(yǎng)基，pH8,100ug/ml?Blasticidin的條件下篩選。高pH可增強Blasticidin的活性，invivogen公司的Fast-Media? Blas培養(yǎng)基可助您得到篩選效果。
哺乳動物細胞

質(zhì)量控制：
純度>95%(HPLC)
經(jīng)細菌和哺乳動物細胞系鑒定活性