產(chǎn)品名稱(chēng)
Other Notes
僅用于生命科學(xué)研究。不可用于診斷。
Quality
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Physical form
Biotin-16-UTP, tetralithium salt, 10mM solution
Application
Biotin-labeled RNA can be used as a hybridization probe for:
? Northern blots
? Southern blots
? Plaque or colony lifts
? RNase protection experiments
? In situ hybridization
? Microarray hybridization[1][2]
General description
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[3] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively. The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate.