產(chǎn)品簡介

DEPC水是用DEPC(diethypyrocarbonate，焦碳酸二乙酯)處理過并經(jīng)高溫高壓消毒的Milli-Q純水。經(jīng)檢測不含RNase、DNase和proteinase。

DEPC水可以用于RNA沉淀的溶解，含有RNA的各種反應(yīng)體系如反轉(zhuǎn)錄、siRNA的退火等，以及其它各種要求無RNase、DNase和proteinase的反應(yīng)體系。

DEPC水不同于DEPC。DEPC水是使用DEPC處理過的水，基本上不含DEPC，99.99%以上是水。

保存條件

室溫保存，一年有效。

注意事項(xiàng)

如果每次的使用量很小，可以適當(dāng)分裝后再使用。

手上通常有RNase，必須戴一次性手套操作，以防RNase污染。

本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于任何其他用途，不得存放于普通住宅內(nèi)。

為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues.

DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases.

Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC. Inactivation of DEPC in this manner yields CO2 and ethanol. Higher concentrations of DEPC are capable of deactivating larger amounts of RNase, but remaining traces or byproducts may inhibit further biochemical reactions such as in vitro translation. Furthermore, chemical modification of RNA such as carboxymethylation is possible when traces of DEPC or its byproducts are present, resulting in impaired recovery of intact RNA even after buffer exchange (after precipitation).