名稱 印度墨汁 印度墨水 Indian Ink, Biotech Grade for Biological Stains

規(guī)格 100mL

產(chǎn)品簡介

墨汁莢膜染色對于觀察新型隱球菌有很好的作用，印度墨汁(India Ink)屬于非常優(yōu)質(zhì)的墨汁，新型隱球菌菌體周圍存在粘多糖莢膜物，用印度墨汁染色法能證明莢膜的存在。染色后新型隱球菌的莢膜取代了墨汁中的膠狀碳粒，菌體被清楚無色透明的暈圈環(huán)所繞著。

級別：BR

生物染色：合格

性狀：黑色液體

用途：生化研究。生物染色劑。

保存：RT,有效期12個月；

自備材料：離心機(jī)；載玻片、蓋玻片

操作步驟(僅供參考)：

1、樣本離心，取沉淀涂于載玻片上，在樣本涂片處滴加India Ink。

2、加蓋蓋玻片，并防止氣泡產(chǎn)生。用手指輕輕壓一下，使樣本與India Ink混合變薄。

3、在低倍鏡下尋找有莢膜的細(xì)菌，并轉(zhuǎn)高倍鏡或油鏡下仔細(xì)辨認(rèn)。

染色結(jié)果

新型隱球菌>>寬厚透亮的莢膜，細(xì)胞壁明顯
背景>>黑色

注意事項

1、已開封India Ink 應(yīng)在6個月內(nèi)用完，每次用后盡量密封好，以免揮發(fā)或變質(zhì)。

2、India Ink存儲時間較長后易產(chǎn)生沉淀，如果鏡下發(fā)現(xiàn)顆粒粗糙者應(yīng)過濾后使用或棄用。

3、目的樣本盡量新鮮。

4、樣本的純度和濃度應(yīng)適宜，濃度過低時在鏡下很難尋找到新型隱球菌。

5、樣本和印度墨汁分布應(yīng)均勻。

Basic India ink is composed of a variety of fine soot, known as lampblack, combined with water to form a liquid.

Microbiologists use India ink to stain a slide containing micro-organisms. The background is stained while the organisms remain clear. This is called a negative stain. India ink, along with other stains, can be used to determine if a cell has a gelatinous capsule. A common application of this procedure in the clinical microbiology laboratory is to confirm the morphology of the encapsulated yeast Cryptococcus spp. which cause cryptococcal meningitis.

Medical researchers use India ink to visualize blood vessels when viewed under a microscope.

Scientists performing Western blotting may use India ink to visualized proteins separated by electrophoresis and transferred to a nitrocellulose or PVDF membrane.

Zoological museum specimens were often tagged in India ink, either directly or on a piece of tracing paper stored along the specimen, because of its durability even when submerged in preservative fluids.

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