名稱 麗春紅染色液 (Ponceau S Staining Solution)
 
規(guī)格 100ml | 500ml
 
存儲(chǔ) 室溫保存，一年有效

產(chǎn)品簡(jiǎn)介 

麗春紅染色液可以用于PVDF膜、硝酸纖維素膜(NC膜；nitrocellulose membrane)和醋酸纖維素膜(cellulose acetate membrane)上的蛋白的檢測(cè)。麗春紅帶負(fù)電荷，可以與帶正電荷的氨基酸殘基結(jié)合，同時(shí)麗春紅也可以與蛋白的非極性區(qū)相結(jié)合，從而形成紅色的條帶。注：麗春紅染色液不適用于尼龍膜上的蛋白的檢測(cè)（詳見(jiàn)附加說(shuō)明）。

麗春紅對(duì)蛋白的染色是可逆的，染色后可以用蒸餾水，PBS或其它適當(dāng)溶液洗去。麗春紅對(duì)蛋白的檢測(cè)下限是250ng。

本染色液為即用型工作液，可以直接使用，不用稀釋。

使用說(shuō)明 

將PVDF膜、硝酸纖維素膜或醋酸纖維素膜浸沒(méi)在麗春紅染色液中，搖動(dòng)5-10分鐘或更長(zhǎng)時(shí)間；取出印跡膜，用蒸餾水，PBS或其它適當(dāng)溶液漂洗2-3次，可出現(xiàn)清晰條帶，記錄結(jié)果；用蒸餾水，PBS或其它適當(dāng)溶液漂洗2-3次，每次3-5分鐘，去除麗春紅，進(jìn)行后續(xù)的WB檢測(cè)。

補(bǔ)充：麗春紅染色液也可以直接用來(lái)染PAGE膠，不過(guò)，在染色之前用甲醇-乙酸溶液浸泡一下PAGE膠，起到固定蛋白的作用。。

附加說(shuō)明 

1）麗春紅染色液可用于PVDF膜，NC膜，醋酸纖維素膜的可逆染色，但不適用于尼龍膜的可逆染色，因?yàn)槟猃埬д姾赡芘c帶負(fù)電荷的麗春紅結(jié)合，不易脫色。

2）為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing. It is also easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.