| 5' | ? | ? | A?? | T?↓ | T?? | A?? | A?? | T?? | ? | 3' | ? |
| 3' | ? | ? | T?? | A?? | A?? | T?↑ | T?? | A?? | ? | 5' | ? |
Thermo Scientific VspI (AseI) restriction enzyme recognizes AT^TAAT sites and cuts best at 37°C in O buffer. See
Reaction Conditions for Restriction Enzymes
for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a
FastDigest enzyme
for rapid DNA digestion. Isoschizomers: AseI, PshBI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features? Superior quality—stringent quality control and industry leading manufacturing process
? Convenient color-coded Five Buffer System
? Includes universal Tango buffer for double-digestions
? BSA premixed in reaction buffers
? Wide selection of restriction endonuclease specificities
Applications? Molecular cloning
? Restriction site mapping
? Genotyping
? Southern blotting
? Restriction fragment length polymorphism (RFLP)
? SNP
Note: For methylation sensitivity, refer to product specifications.
關鍵字： VspI (AseI) (10 U/μL);分子生物學
北京索萊寶科技有限公司是一家集研發(fā)、生產、銷售、服務于一體的高科技生物企業(yè)。技術推廣;技術轉讓；技術咨詢；技術服務；生物試劑技術開發(fā)；銷售生物試劑盒、實驗室專用化學試劑、實驗室專用設備、辦公用品、化工產品（不含危險化學品）、計算機軟件及輔助設備；貨物進出口、技術進出口、代理進出口；委托加工生產生物制品、生化制品、化工產品（不含危險化學品）；生產生物試劑盒；批發(fā)第一類醫(yī)療器械、第二類醫(yī)療器械；批發(fā)第三類醫(yī)療器械。