Recombinant Mouse Apolipoprotein E is produced by our Mammalian expression system and the target gene encoding Glu19-Gln311 is expressed with a 6His tag at the C-terminus.
Background
Apolipoprotein E (Apo-E), is a member of the apolipoprotein A1/A4/E family. ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. APOE may function in mediating the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. APOE is usually secreted in plasma. Phosphorylation sites are present in the extracellular medium.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
關(guān)鍵字： Recombinant Mouse Apolipoprotein E/Apo-E/APOE;細(xì)胞生物學(xué)
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